Implementation of Random Bacterial Genomic DNA Microarray Chip (RBGDMC) for Screening of Dominant Bacteria in Complex Cultures
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Byoung Chan | - |
dc.contributor.author | Park, Ji Hyun | - |
dc.contributor.author | Gu, Man Bock | - |
dc.date.accessioned | 2021-09-07T22:44:49Z | - |
dc.date.available | 2021-09-07T22:44:49Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2010-12 | - |
dc.identifier.issn | 0273-2289 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/115278 | - |
dc.description.abstract | The random bacterial genomic DNA microarray chip (RBGDMC), which was fabricated using random genomic DNA fragments obtained from the fragmentation of bacterial genome by using four different pairs of restriction enzymes, was found to discriminate bacterial species in the same genus and resulted in the determination of dominant bacteria in enriched cultures. The identification of a dominant bacterial species was successfully conducted in the co-culture of three different bacteria using the RBGDMC. In addition, the analysis of the chip data could confirm if any of the selected bacteria is the most abundant or if some bacteria were enriched and became the dominant species within the consortium after the samples were prepared from the repeated cultures of real sludge in a complex medium. This study shows the successful implementation of the RBGDMC for the identification and monitoring of dominant bacteria in complex environmental bacterial communities simply without any PCR amplification of the target nucleic acids. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | HUMANA PRESS INC | - |
dc.subject | IN-SITU HYBRIDIZATION | - |
dc.subject | 16S RIBOSOMAL-RNA | - |
dc.subject | OLIGONUCLEOTIDE MICROARRAY | - |
dc.subject | MICROBIAL COMMUNITY | - |
dc.subject | ACTIVATED-SLUDGE | - |
dc.subject | GENES | - |
dc.subject | PCR | - |
dc.subject | DIVERSITY | - |
dc.subject | PROBES | - |
dc.subject | IDENTIFICATION | - |
dc.title | Implementation of Random Bacterial Genomic DNA Microarray Chip (RBGDMC) for Screening of Dominant Bacteria in Complex Cultures | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Gu, Man Bock | - |
dc.identifier.doi | 10.1007/s12010-010-9002-4 | - |
dc.identifier.scopusid | 2-s2.0-78149468873 | - |
dc.identifier.wosid | 000284295000015 | - |
dc.identifier.bibliographicCitation | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.162, no.8, pp.2284 - 2293 | - |
dc.relation.isPartOf | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | - |
dc.citation.title | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | - |
dc.citation.volume | 162 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 2284 | - |
dc.citation.endPage | 2293 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | IN-SITU HYBRIDIZATION | - |
dc.subject.keywordPlus | 16S RIBOSOMAL-RNA | - |
dc.subject.keywordPlus | OLIGONUCLEOTIDE MICROARRAY | - |
dc.subject.keywordPlus | MICROBIAL COMMUNITY | - |
dc.subject.keywordPlus | ACTIVATED-SLUDGE | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordPlus | DIVERSITY | - |
dc.subject.keywordPlus | PROBES | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordAuthor | Random fragmentation | - |
dc.subject.keywordAuthor | Genomic DNA | - |
dc.subject.keywordAuthor | DNA microarray chip | - |
dc.subject.keywordAuthor | Bacteria identification | - |
dc.subject.keywordAuthor | Complex culture | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.