Enhancement of DC vaccine potency by activating the PI3K/AKT pathway with a small interfering RNA targeting PTEN
DC Field | Value | Language |
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dc.contributor.author | Kim, Jin Hee | - |
dc.contributor.author | Kang, Tae Heung | - |
dc.contributor.author | Noh, Kyung Hee | - |
dc.contributor.author | Kim, Seok-Ho | - |
dc.contributor.author | Lee, Young-Ho | - |
dc.contributor.author | Kim, Keon Woo | - |
dc.contributor.author | Bae, Hyun Cheol | - |
dc.contributor.author | Ahn, Ye-Hyeon | - |
dc.contributor.author | Choi, Eun Young | - |
dc.contributor.author | Kim, Jin-Seok | - |
dc.contributor.author | Lee, Kyung-Mi | - |
dc.contributor.author | Kim, Tae Woo | - |
dc.date.accessioned | 2021-09-07T22:49:51Z | - |
dc.date.available | 2021-09-07T22:49:51Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2010-11-30 | - |
dc.identifier.issn | 0165-2478 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/115306 | - |
dc.description.abstract | Dendritic cell (DC)-based cancer vaccines have become Important as an immunotherapeutics in generating anti-tumor immune responses Due to a short lifespan of DCs however clinical application of current DC vaccines has been limited Recently activation of AKT/protein kinase B (PKB) a major effector of phosphatidylinositol 3-kinase (PI3K) has been reported as a critical factor in both activation and survival of DCs We here improved the potency of a DC vaccine with a small interfering RNA (siRNA) targeting phosphatase and tensin homologue (PTEN) which is known to be a central negative regulator of the PI3K/AKT signal transduction cascade Down-regulation of PTEN in DCs resulted in AKT dependent maturation which in turn caused a significant up-regulation of surface expression in co-stimulatory molecules and the chemokine receptor CCR7 leading to an increase of in vitro T cell activation activity and in vivo migration to a draining lymph node respectively Moreover these PTEN siRNA-transfected DCs (DC/siPTEN) acquired an Increased survival from the apoptotic death caused by GM-CSF deprivation or antigen-specific CD8(+) T cell killing Most importantly DC/siPTEN generated more tumor antigen-specific CD8(+) T cells and stronger anti-tumor effects in vaccinated mice than did control DCs (DC/siGFP) Thus our data indicate that manipulation of the PI3K/AKT pathway via siRNA system could improve the efficacy of a DC-based tumor vaccine (C) 2010 Elsevier B V All rights reserved | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.subject | SIGNAL-TRANSDUCTION PATHWAYS | - |
dc.subject | DENDRITIC CELL LIFE | - |
dc.subject | BH3-ONLY PROTEINS | - |
dc.subject | SURVIVAL | - |
dc.subject | KINASE | - |
dc.subject | APOPTOSIS | - |
dc.subject | STRATEGY | - |
dc.subject | LIGAND | - |
dc.subject | BAX | - |
dc.title | Enhancement of DC vaccine potency by activating the PI3K/AKT pathway with a small interfering RNA targeting PTEN | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Tae Woo | - |
dc.identifier.doi | 10.1016/j.imlet.2010.08.008 | - |
dc.identifier.scopusid | 2-s2.0-78049306941 | - |
dc.identifier.wosid | 000284657400006 | - |
dc.identifier.bibliographicCitation | IMMUNOLOGY LETTERS, v.134, no.1, pp.47 - 54 | - |
dc.relation.isPartOf | IMMUNOLOGY LETTERS | - |
dc.citation.title | IMMUNOLOGY LETTERS | - |
dc.citation.volume | 134 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 47 | - |
dc.citation.endPage | 54 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION PATHWAYS | - |
dc.subject.keywordPlus | DENDRITIC CELL LIFE | - |
dc.subject.keywordPlus | BH3-ONLY PROTEINS | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | STRATEGY | - |
dc.subject.keywordPlus | LIGAND | - |
dc.subject.keywordPlus | BAX | - |
dc.subject.keywordAuthor | Dendritic cell | - |
dc.subject.keywordAuthor | Immunotherapy | - |
dc.subject.keywordAuthor | siRNA | - |
dc.subject.keywordAuthor | AKT | - |
dc.subject.keywordAuthor | PI3K | - |
dc.subject.keywordAuthor | PTEN | - |
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