Performance of beta-galactosidase pretreated with lactose to prevent activity loss during the enzyme immobilisation process
DC Field | Value | Language |
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dc.contributor.author | Song, Yoon Seok | - |
dc.contributor.author | Lee, Jong Ho | - |
dc.contributor.author | Kang, Seong Woo | - |
dc.contributor.author | Kim, Seung Wook | - |
dc.date.accessioned | 2021-09-07T23:00:52Z | - |
dc.date.available | 2021-09-07T23:00:52Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2010-11-01 | - |
dc.identifier.issn | 0308-8146 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/115349 | - |
dc.description.abstract | In this study, Kluyveromyces lactis beta-galactosidase was pretreated with lactose to prevent loss of activity during the immobilisation process, and glutaraldehyde was used as a linker to immobilise beta-galactosidase on the surface of a silica gel. The pretreatment of beta-galactosidase strongly improved its activity after immobilisation. Specifically, the activity of pretreated immobilised beta-galactosidase was 2.6 times greater than that of non-pretreated immobilised beta-galactosidase. The optimal temperature, pH and ionic strength of buffer for pretreated immobilised beta-galactosidase were 37 degrees C, pH 7.5 and 20 mM potassium phosphate buffer, respectively. These values were shifted by 5 degrees C and pH by 0.5 when compared to the soluble beta-galactosidase. Moreover, the pretreated immobilised beta-galactosidase showed a better reusability than did non-pretreated immobilised beta-galactosidase, with 63.9% of its original activity being retained after 10 reuses. (C) 2010 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | KLUYVEROMYCES-LACTIS | - |
dc.subject | LIPASE IMMOBILIZATION | - |
dc.subject | SUPPORT | - |
dc.subject | SILICA | - |
dc.title | Performance of beta-galactosidase pretreated with lactose to prevent activity loss during the enzyme immobilisation process | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Seung Wook | - |
dc.identifier.doi | 10.1016/j.foodchem.2010.04.043 | - |
dc.identifier.scopusid | 2-s2.0-77952585408 | - |
dc.identifier.wosid | 000279390900001 | - |
dc.identifier.bibliographicCitation | FOOD CHEMISTRY, v.123, no.1, pp.1 - 5 | - |
dc.relation.isPartOf | FOOD CHEMISTRY | - |
dc.citation.title | FOOD CHEMISTRY | - |
dc.citation.volume | 123 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 5 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalResearchArea | Nutrition & Dietetics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Nutrition & Dietetics | - |
dc.subject.keywordPlus | KLUYVEROMYCES-LACTIS | - |
dc.subject.keywordPlus | LIPASE IMMOBILIZATION | - |
dc.subject.keywordPlus | SUPPORT | - |
dc.subject.keywordPlus | SILICA | - |
dc.subject.keywordAuthor | beta-Galactosidase | - |
dc.subject.keywordAuthor | Pretreatment | - |
dc.subject.keywordAuthor | Lactose | - |
dc.subject.keywordAuthor | Immobilisation | - |
dc.subject.keywordAuthor | Silica gel | - |
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