Neuroglobin protects neurons against oxidative stress in global ischemia
DC Field | Value | Language |
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dc.contributor.author | Li, Richard Changxun | - |
dc.contributor.author | Guo, Shang Zhi | - |
dc.contributor.author | Lee, Seung Kwan | - |
dc.contributor.author | Gozal, David | - |
dc.date.accessioned | 2021-09-07T23:25:19Z | - |
dc.date.available | 2021-09-07T23:25:19Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2010-11 | - |
dc.identifier.issn | 0271-678X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/115484 | - |
dc.description.abstract | Neuroglobin (Ngb) is a recently discovered globin that affords protection against hypoxic/ischemic-induced cell injury in brain. Hypoxic/ischemic injury is associated with accumulation of reactive oxygen species (ROS) and/or reactive nitrogen species (RNS). In previous studies, we found that Ngb has antioxidative properties, and protects PC-12 cells against hypoxia-and beta-amyloid-induced cell death. To further delineate the potential role of Ngb in protection against cerebral ischemia-reperfusion injury in vivo, we developed a transgenic mouse line that overexpresses Ngb. Hippocampal ischemia-reperfusion injury was induced by a 10-minute bilateral occlusion of the common carotid arteries, and the animal brains were assessed 3 days later. CA1 neural injury was determined by cresyl violet staining. Lipid peroxidation was assessed using a malonyldialdehyde assay kit, whereas ROS/RNS accumulation was determined by Het staining in the CA1 hippocampal region. Hippocampal Ngb mRNA and protein expressions were assessed by reverse transcriptase-PCR and western blotting, respectively. Neuroglobin was successfully overexpressed in the hippocampus of Ngb transgenic mice. After ischemia-reperfusion, CA1 ROS/RNS production and lipid peroxidation were markedly decreased in Ngb transgenic mice compared with wild-type mice. Furthermore, CA1 neuronal injury was also markedly reduced. Thus, Ngb may confer protection against ischemia-reperfusion injury in the brain through its intrinsic antioxidant properties. Journal of Cerebral Blood Flow & Metabolism (2010) 30, 1874-1882; doi:10.1038/jcbfm.2010.90; published online 23 June 2010 | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SAGE PUBLICATIONS INC | - |
dc.subject | DISSOCIATION INHIBITOR ACTIVITY | - |
dc.subject | REACTIVE OXYGEN RADICALS | - |
dc.subject | FOCAL CEREBRAL-ISCHEMIA | - |
dc.subject | TRANSGENIC MICE | - |
dc.subject | CELL INJURY | - |
dc.subject | PC12 CELLS | - |
dc.subject | RAT-BRAIN | - |
dc.subject | CYTOGLOBIN | - |
dc.subject | EXPRESSION | - |
dc.subject | OVEREXPRESSION | - |
dc.title | Neuroglobin protects neurons against oxidative stress in global ischemia | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Seung Kwan | - |
dc.identifier.doi | 10.1038/jcbfm.2010.90 | - |
dc.identifier.scopusid | 2-s2.0-78049458153 | - |
dc.identifier.wosid | 000283736300011 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, v.30, no.11, pp.1874 - 1882 | - |
dc.relation.isPartOf | JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM | - |
dc.citation.title | JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM | - |
dc.citation.volume | 30 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1874 | - |
dc.citation.endPage | 1882 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Endocrinology & Metabolism | - |
dc.relation.journalResearchArea | Hematology | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalWebOfScienceCategory | Endocrinology & Metabolism | - |
dc.relation.journalWebOfScienceCategory | Hematology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.subject.keywordPlus | DISSOCIATION INHIBITOR ACTIVITY | - |
dc.subject.keywordPlus | REACTIVE OXYGEN RADICALS | - |
dc.subject.keywordPlus | FOCAL CEREBRAL-ISCHEMIA | - |
dc.subject.keywordPlus | TRANSGENIC MICE | - |
dc.subject.keywordPlus | CELL INJURY | - |
dc.subject.keywordPlus | PC12 CELLS | - |
dc.subject.keywordPlus | RAT-BRAIN | - |
dc.subject.keywordPlus | CYTOGLOBIN | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | OVEREXPRESSION | - |
dc.subject.keywordAuthor | global ischemia | - |
dc.subject.keywordAuthor | hippocampus | - |
dc.subject.keywordAuthor | neuroglobin | - |
dc.subject.keywordAuthor | neuron | - |
dc.subject.keywordAuthor | oxidative stress | - |
dc.subject.keywordAuthor | transgenic mouse | - |
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