Disposable amperometric biosensor based on nanostructured bacteriophages for glucose detection
DC Field | Value | Language |
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dc.contributor.author | Kang, Yu Ri | - |
dc.contributor.author | Hwang, Kyung Hoon | - |
dc.contributor.author | Kim, Ju Hwan | - |
dc.contributor.author | Nam, Chang Hoon | - |
dc.contributor.author | Kim, Soo Won | - |
dc.date.accessioned | 2021-09-07T23:44:34Z | - |
dc.date.available | 2021-09-07T23:44:34Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2010-10 | - |
dc.identifier.issn | 0957-0233 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/115572 | - |
dc.description.abstract | The selection of electrode material profoundly influences biosensor science and engineering, as it heavily influences biosensor sensitivity. Here we propose a novel electrochemical detection method using a working electrode consisting of bio-nanowires from genetically modified filamentous phages and nanoparticles. fd-tet p8MMM filamentous phages displaying a three-methionine (MMM) peptide on the major coat protein pVIII (designated p8MMM phages) were immobilized on the active area of an electrochemical sensor through physical adsorption and chemical bonding. Bio-nanowires composed of p8MMM phages and silver nanoparticles facilitated sensitive, rapid and selective detection of particular molecules. We explored whether the composite electrode with bio-nanowires was an effective platform to detect the glucose oxidase. The current response of the bio-nanowire sensor was high at various glucose concentrations (0.1 mu M-0.1 mM). This method provides a considerable advantage to demonstrate analyte detection over low concentration ranges. Especially, phage-enabled bio-nanowires can serve as receptors with high affinity and specificity for the detection of particular biomolecules and provide a convenient platform for designing site-directed multifunctional scaffolds based on bacteriophages and may serve as a simple method for label-free detection. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | IOP PUBLISHING LTD | - |
dc.subject | PHAGE DISPLAY | - |
dc.subject | ELECTRODE | - |
dc.subject | GOLD | - |
dc.subject | CONSTRUCTION | - |
dc.subject | ANTIBODY | - |
dc.subject | FABRICATION | - |
dc.subject | SELECTION | - |
dc.subject | PARTICLE | - |
dc.subject | LIBRARY | - |
dc.subject | BINDING | - |
dc.title | Disposable amperometric biosensor based on nanostructured bacteriophages for glucose detection | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Soo Won | - |
dc.identifier.doi | 10.1088/0957-0233/21/10/105804 | - |
dc.identifier.scopusid | 2-s2.0-78149396323 | - |
dc.identifier.wosid | 000281787100056 | - |
dc.identifier.bibliographicCitation | MEASUREMENT SCIENCE AND TECHNOLOGY, v.21, no.10 | - |
dc.relation.isPartOf | MEASUREMENT SCIENCE AND TECHNOLOGY | - |
dc.citation.title | MEASUREMENT SCIENCE AND TECHNOLOGY | - |
dc.citation.volume | 21 | - |
dc.citation.number | 10 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Engineering, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.subject.keywordPlus | PHAGE DISPLAY | - |
dc.subject.keywordPlus | ELECTRODE | - |
dc.subject.keywordPlus | GOLD | - |
dc.subject.keywordPlus | CONSTRUCTION | - |
dc.subject.keywordPlus | ANTIBODY | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.subject.keywordPlus | SELECTION | - |
dc.subject.keywordPlus | PARTICLE | - |
dc.subject.keywordPlus | LIBRARY | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordAuthor | disposable analytical system | - |
dc.subject.keywordAuthor | biosensor application | - |
dc.subject.keywordAuthor | bacteriophage | - |
dc.subject.keywordAuthor | label-free detection | - |
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