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Increased Ethanol Resistance in Ethanolic Escherichia coli by Insertion of Heat-shock Genes BEM1 and SOD2 from Saccharomyces cerevisiae

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dc.contributor.authorLee, Soo Jin-
dc.contributor.authorOh, Eun Kyoung-
dc.contributor.authorOh, Young Hoon-
dc.contributor.authorWon, Jong In-
dc.contributor.authorHan, Sung Ok-
dc.contributor.authorLee, Jin Won-
dc.date.accessioned2021-09-08T00:25:35Z-
dc.date.available2021-09-08T00:25:35Z-
dc.date.issued2010-09-
dc.identifier.issn1226-8372-
dc.identifier.issn1976-3816-
dc.identifier.urihttps://scholar.korea.ac.kr/handle/2021.sw.korea/115741-
dc.description.abstractEthanol is generally toxic to microorganisms, and intracellular and extracellular accumulation of ethanol inhibits cell growth and metabolism. In this study, pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB) were cloned into pET-32a vector and then introduced into E. coli BL21 to produce ethanol. Heat shock genes (BEM1 and SOD2) from Saccharomyces cerevisiae were inserted into recombinant ethanolic E. coli using pET28_a vector to improve ethanol shock resistance. Three different strains were constructed: Ethanolic E. (adhB and pdc genes inserted using pET32_a vector), BEM1 gene-inserted E. coli (BEM1 inserted using pET_28a), and SOD2-inserted E. coli (SOD2 inserted using pET28_a). Construction of these three different strains allowed comparison of the functions of these heat shock genes as well as their roles in ethanol tolerance. The toxicity of ethanol in recombinant ethanolic E. coli was tested by measuring cell growth in response to various ethanol concentrations. The results show that SOD2-inserted E. coli showed higher ethanol resistance than ethanolic E. coli.-
dc.format.extent7-
dc.language영어-
dc.language.isoENG-
dc.publisherKOREAN SOC BIOTECHNOLOGY & BIOENGINEERING-
dc.titleIncreased Ethanol Resistance in Ethanolic Escherichia coli by Insertion of Heat-shock Genes BEM1 and SOD2 from Saccharomyces cerevisiae-
dc.typeArticle-
dc.publisher.location대한민국-
dc.identifier.doi10.1007/s12257-009-3060-x-
dc.identifier.scopusid2-s2.0-78649696298-
dc.identifier.wosid000283819900009-
dc.identifier.bibliographicCitationBIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.15, no.5, pp 770 - 776-
dc.citation.titleBIOTECHNOLOGY AND BIOPROCESS ENGINEERING-
dc.citation.volume15-
dc.citation.number5-
dc.citation.startPage770-
dc.citation.endPage776-
dc.type.docTypeArticle-
dc.identifier.kciidART001490553-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.description.journalRegisteredClasskci-
dc.relation.journalResearchAreaBiotechnology & Applied Microbiology-
dc.relation.journalWebOfScienceCategoryBiotechnology & Applied Microbiology-
dc.subject.keywordPlusALCOHOL DEHYDROGENASE-II-
dc.subject.keywordPlusZYMOMONAS-MOBILIS-
dc.subject.keywordPlusPYRUVATE DECARBOXYLASE-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusTOLERANCE-
dc.subject.keywordPlusIDENTIFICATION-
dc.subject.keywordPlusFERMENTATION-
dc.subject.keywordPlusINHIBITION-
dc.subject.keywordPlusMECHANISM-
dc.subject.keywordPlusMUTANTS-
dc.subject.keywordAuthorbioethanol-
dc.subject.keywordAuthorheat shock genes (SOD2, BEM1)-
dc.subject.keywordAuthorethanol resistance-
dc.subject.keywordAuthorEscherichia coli-
dc.subject.keywordAuthorSaccharomyces cerevisiae-
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