High-sensitivity detection of oxytetracycline using light scattering agglutination assay with aptasensor
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Keesung | - |
dc.contributor.author | Gu, Man-Bock | - |
dc.contributor.author | Kang, Do-Hyun | - |
dc.contributor.author | Park, Jee-Won | - |
dc.contributor.author | Song, In-Hong | - |
dc.contributor.author | Jung, Ho-Sup | - |
dc.contributor.author | Suh, Kahp-Yang | - |
dc.date.accessioned | 2021-09-08T00:32:10Z | - |
dc.date.available | 2021-09-08T00:32:10Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2010-09 | - |
dc.identifier.issn | 0173-0835 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/115776 | - |
dc.description.abstract | We present an aptamer-based biosensor (aptasensor) for rapid and high-sensitive detection of oxytetracycline (OTC) antibiotic in PBS inside a Y-channel PDMS microfluidic device. The detection was made by real-time monitoring of the agglutination assay of ssDNA aptamer-conjugated polystyrene latex microspheres with proximity optical fibers. The agglutination assay was performed with serially diluted OTC antibiotic solutions using highly carboxylated polystyrene particles of 920 nm diameter conjugated with OTC-binding ssDNA aptamer. Proximity optical fibers were used to measure the increase in 450 forward light scattering of the aggregated particles by fixing them around the viewing cell of the device with stable angle and distance to the detector. The detection limit was around 100 ppb for the current aptasensor system with the detection time less than 3 min. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | IMMUNOAGGLUTINATION ASSAY | - |
dc.subject | APTAMERS | - |
dc.title | High-sensitivity detection of oxytetracycline using light scattering agglutination assay with aptasensor | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Gu, Man-Bock | - |
dc.identifier.doi | 10.1002/elps.201000217 | - |
dc.identifier.scopusid | 2-s2.0-77956892661 | - |
dc.identifier.wosid | 000283389900014 | - |
dc.identifier.bibliographicCitation | ELECTROPHORESIS, v.31, no.18, pp.3115 - 3120 | - |
dc.relation.isPartOf | ELECTROPHORESIS | - |
dc.citation.title | ELECTROPHORESIS | - |
dc.citation.volume | 31 | - |
dc.citation.number | 18 | - |
dc.citation.startPage | 3115 | - |
dc.citation.endPage | 3120 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | IMMUNOAGGLUTINATION ASSAY | - |
dc.subject.keywordPlus | APTAMERS | - |
dc.subject.keywordAuthor | Agglutination assay | - |
dc.subject.keywordAuthor | Aptamer | - |
dc.subject.keywordAuthor | Microfluidic device | - |
dc.subject.keywordAuthor | Oxytetracycline | - |
dc.subject.keywordAuthor | Static light scattering | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.