Concentration of Arginine and Optimal Time of Hypertonic Saline in Restoration of T-Cell Dysfunction
DC Field | Value | Language |
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dc.contributor.author | Moon, Sung-Woo | - |
dc.contributor.author | Choi, Sung-Hyuk | - |
dc.contributor.author | Cho, Han-Jin | - |
dc.contributor.author | Yun, Young-Hoon | - |
dc.contributor.author | Kim, Jung-Yoen | - |
dc.contributor.author | Hong, Yun-Sik | - |
dc.contributor.author | Costantini, Todd | - |
dc.contributor.author | Bansal, Vishal | - |
dc.date.accessioned | 2021-09-08T00:41:26Z | - |
dc.date.available | 2021-09-08T00:41:26Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2010-09 | - |
dc.identifier.issn | 0022-4804 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/115823 | - |
dc.description.abstract | Background. Hypertonic saline (HS) restores prostaglandin E-2 (PGE(2))-induced T-cell suppression in the presence of 1100 mu M arginine. However, under arginine-free culture conditions, HS dose not restore T-cell proliferation. Therefore, we wanted to determine if HS can restore PGE(2)-induced T-cell suppression in the presence of 80 mu M of arginine, the physiologically relevant arginine concentration. We also wanted to determine the concentration of arginine that induces HS restoration of PGE(2)-suppressed T-cell proliferation and whether HS restoration of T-cell dysfunction is dependent on the injection time of HS. Materials and Methods. Jurkat cells were cultured in media containing 0, 40, 80, 400, 800, or 1100 mu M arginine. In both the PGE(2)-stimulated and HS-treated group, we measured cell proliferation using MTT assay and arginase activity. We also measured cell proliferation relative to HS injection time. Results. In 80 mu M arginine, HS did not restore Jurkat cell proliferation that had been suppressed by PGE2. Increased concentrations of arginine in the media increased MTT cell proliferation. In 800 mu M arginine media, HS restored PGE2-suppressed Jurkat cell proliferation to normal. HS restored PGE(2)-suppressed Jurkat cell proliferation when it was added at 2 h, similar to at same time and 1 h after PGE(2) stimulation. Conclusions. In order to restore PGE(2)-suppressed Jurkat cell proliferation, HS requires at least 800 mu M arginine. HS restored PGE(2)-suppressed Jurkat cell proliferation even though HS was added at 2 h after PGE(2) stimulation. (C) 2010 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | INDUCED IMMUNOSUPPRESSION | - |
dc.subject | DECREASES SUSCEPTIBILITY | - |
dc.subject | PROSTAGLANDIN E(2) | - |
dc.subject | ARGINASE | - |
dc.subject | RESUSCITATION | - |
dc.subject | SUPPRESSION | - |
dc.subject | INHIBITION | - |
dc.subject | HEMORRHAGE | - |
dc.subject | TRAUMA | - |
dc.subject | EXPRESSION | - |
dc.title | Concentration of Arginine and Optimal Time of Hypertonic Saline in Restoration of T-Cell Dysfunction | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Moon, Sung-Woo | - |
dc.contributor.affiliatedAuthor | Choi, Sung-Hyuk | - |
dc.contributor.affiliatedAuthor | Cho, Han-Jin | - |
dc.contributor.affiliatedAuthor | Hong, Yun-Sik | - |
dc.identifier.doi | 10.1016/j.jss.2010.03.036 | - |
dc.identifier.scopusid | 2-s2.0-77955919532 | - |
dc.identifier.wosid | 000291445200003 | - |
dc.identifier.bibliographicCitation | JOURNAL OF SURGICAL RESEARCH, v.163, no.1, pp.E17 - E22 | - |
dc.relation.isPartOf | JOURNAL OF SURGICAL RESEARCH | - |
dc.citation.title | JOURNAL OF SURGICAL RESEARCH | - |
dc.citation.volume | 163 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | E17 | - |
dc.citation.endPage | E22 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Surgery | - |
dc.relation.journalWebOfScienceCategory | Surgery | - |
dc.subject.keywordPlus | INDUCED IMMUNOSUPPRESSION | - |
dc.subject.keywordPlus | DECREASES SUSCEPTIBILITY | - |
dc.subject.keywordPlus | PROSTAGLANDIN E(2) | - |
dc.subject.keywordPlus | ARGINASE | - |
dc.subject.keywordPlus | RESUSCITATION | - |
dc.subject.keywordPlus | SUPPRESSION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | HEMORRHAGE | - |
dc.subject.keywordPlus | TRAUMA | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | hypertonic saline | - |
dc.subject.keywordAuthor | arginine | - |
dc.subject.keywordAuthor | time | - |
dc.subject.keywordAuthor | trauma | - |
dc.subject.keywordAuthor | T-cells | - |
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