Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Production of minicellulosomes from Clostridium cellulovorans for the fermentation of cellulosic ethanol using engineered recombinant Saccharomyces cerevisiae

Authors
Hyeon, Jeong-eunYu, Kyung-OkSuh, Dong JinSuh, Young-WoongLee, Sung EunLee, JinwonHan, Sung Ok
Issue Date
9월-2010
Publisher
WILEY-BLACKWELL
Keywords
minicellulosome; Clostridium cellulovorans; Saccharomyces cerevisiae; cellulose-binding domain; consolidated bioprocessing; ethanol fermentation
Citation
FEMS MICROBIOLOGY LETTERS, v.310, no.1, pp.39 - 47
Indexed
SCIE
SCOPUS
Journal Title
FEMS MICROBIOLOGY LETTERS
Volume
310
Number
1
Start Page
39
End Page
47
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/115845
DOI
10.1111/j.1574-6968.2010.02035.x
ISSN
0378-1097
Abstract
Saccharomyces cerevisiae was engineered for assembly of minicellulosomes by heterologous expression of a recombinant scaffolding protein from Clostridium cellulovorans and a chimeric endoglucanase E from Clostridium thermocellum. The chimeric endoglucanase E fused with the dockerin domain of endoglucanase B from C. cellulovorans was assembled with the recombinant scaffolding protein. The resulting strain was able to ferment amorphous cellulose [carboxymethyl-cellulose (CMC)] into ethanol with the aid of beta-glucosidase 1 produced from Saccharomycopsis fibuligera. The minicellulosomes assembled in vivo retained the synergistic effect for cellulose hydrolysis. The minicellulosomes containing the cellulose-binding domain were purified by crystalline cellulose affinity in a single step. In the fermentation test at 10 g L-1 initial CMC, approximately 3.45 g L-1 ethanol was produced after 16 h. The yield (in grams of ethanol produced per substrate) was 0.34 g g-1 from CMC. This result indicates that a one-step processing of cellulosic biomass in a consolidated bioprocessing configuration is technically feasible by recombinant yeast cells expressing functional minicellulosomes.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Biotechnology > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE