Elevated O-Linked N-Acetylglucosamine Correlated with Reduced Sp1 Cooperative DNA Binding with Its Collaborating Factors in Vivo
DC Field | Value | Language |
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dc.contributor.author | Lim, Kihong | - |
dc.contributor.author | Chang, Hyo-Ihl | - |
dc.date.accessioned | 2021-09-08T01:04:49Z | - |
dc.date.available | 2021-09-08T01:04:49Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2010-08 | - |
dc.identifier.issn | 0916-8451 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/115913 | - |
dc.description.abstract | O-Linked N-acetylglucosamine (O-GIcNAc), a single GIcNAc modification of proteins, is abundant in nucleocytoplasmic proteins of eukaryotes. Most nuclear transcriptional regulator proteins carry O-GlcNAc, implicating O-GlcNAc in gene regulation. This study suggested the possibility that O-GIcNAc regulates cooperative binding of Sp1 and its collaborating transcription factors, Oct1 and Elf-1, onto DNA templates in vivo. Chromatin immunoprecipitation assays on cells in which O-GlcNAc was modulated pharmacologically revealed that Sp1-Oct1- and Sp1-Elf-1-paired occupancies of previously known target promoter regions were suppressed by elevated O-GIcNAc modification. Since these pairs of transcription factors bind the target promoters cooperatively and DNA binding of Sp1 alone is not affected by O-GIcNAc, our results imply that O-GlcNAc weakens the DNA binding of Sp1 and its cooperative binding partners by inhibiting stable interaction on DNA templates. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.subject | GLCNAC INHIBITS INTERACTION | - |
dc.subject | TRANSCRIPTION FACTORS SP1 | - |
dc.subject | INSULIN-RESISTANCE | - |
dc.subject | NUCLEOCYTOPLASMIC PROTEINS | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | GLYCOSYLATION | - |
dc.subject | STREPTOZOTOCIN | - |
dc.subject | ACTIVATION | - |
dc.subject | CELLS | - |
dc.subject | PHOSPHORYLATION | - |
dc.title | Elevated O-Linked N-Acetylglucosamine Correlated with Reduced Sp1 Cooperative DNA Binding with Its Collaborating Factors in Vivo | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chang, Hyo-Ihl | - |
dc.identifier.doi | 10.1271/bbb.100289 | - |
dc.identifier.scopusid | 2-s2.0-77956027836 | - |
dc.identifier.wosid | 000281722200026 | - |
dc.identifier.bibliographicCitation | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.74, no.8, pp.1668 - 1672 | - |
dc.relation.isPartOf | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | - |
dc.citation.title | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | - |
dc.citation.volume | 74 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1668 | - |
dc.citation.endPage | 1672 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | GLCNAC INHIBITS INTERACTION | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTORS SP1 | - |
dc.subject.keywordPlus | INSULIN-RESISTANCE | - |
dc.subject.keywordPlus | NUCLEOCYTOPLASMIC PROTEINS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | GLYCOSYLATION | - |
dc.subject.keywordPlus | STREPTOZOTOCIN | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordAuthor | cooperative DNA binding | - |
dc.subject.keywordAuthor | Elf-1 | - |
dc.subject.keywordAuthor | O-linked N-acetylglucosamine | - |
dc.subject.keywordAuthor | Oct1 | - |
dc.subject.keywordAuthor | Sp1 | - |
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