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Immunological Responses Induced by asd and wzy/asd Mutant Strains of Salmonella enterica serovar Typhimurium in BALB/c Mice

Authors
Piao, Hong HuaTam, Vo Thi MinhNa, Hee SamKim, Hyun JuRyu, Phil YoulKim, Soo YoungRhee, Joon HaengChoy, Hyon E.Kim, Suhng WookHong, Yeongjin
Issue Date
8월-2010
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Keywords
Salmonella enterica serovar Typhimurium; live vaccine; immune responses; wzy and asd
Citation
JOURNAL OF MICROBIOLOGY, v.48, no.4, pp.486 - 495
Indexed
SCIE
SCOPUS
KCI
Journal Title
JOURNAL OF MICROBIOLOGY
Volume
48
Number
4
Start Page
486
End Page
495
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/115965
DOI
10.1007/s12275-010-0023-z
ISSN
1225-8873
Abstract
Attenuated bacteria have long been developed as vaccine candidates but can have some disadvantages, such as the potential for damage to immune organs due to insufficient clearance. To minimize these disadvantages, we generated Salmonella enterica serovar Typhimurium mutants SHJ2104 (asd::cm)and HTSaYA (wzy::km, asd::cm). The wzy gene codes for the O-antigen polymerase, which is involved in lipopolysaccharide (LPS) biosynthesis, and asd codes for aspartate B-semialdehyde dehydrogenase, which participates in cell wall formation. The strains synthesized LPS with a short-chain length, and showed lower cytotoxicity and reduced intracellular proliferation in animal cells compared to wild-type bacteria. After oral infection, the mutants were cleared in immune tissues, including the Peyer's patch, mesenteric lymph node, and spleen, within 5 days. The LD50 of the mutants in Balb/c mice was estimated to be 106 higher than wild-type bacteria when administered either via an oral or i.p. route, indicating that the two strains are highly attenuated. To compare the immune response to and protective effects of the mutants against wild-type bacterial infection, we inoculated the mutants into mice via an oral (1x10(10) CFU) or i.p. (1 x 10(7) CFU) route once or twice at a two week interval. All immune responses, such as serum IgG and secretory IgA levels, cytokine production, and delayed hypersensitivity, were highly induced by two rounds of immunization. HTSaYA and SHJ2104 induced similar immune responses, and mice immunized with HTSaYA or SHJ2104 via an i.p. route were protected against wild-type Salmonella infection even at 100-fold of the LD50 (5x10(6) CFU). Taken together, these data indicate that HTSaYA and SHJ2104 could be developed as live attenuated Salmonella vaccine candidates.
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보건과학대학 (보건환경융합과학부)
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