Notochordal Cells Influence Gene Expression of Inflammatory Mediators of Annulus Fibrosus Cells in Proinflammatory Cytokines Stimulation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Moon, Hong Joo | - |
dc.contributor.author | Joe, Noon | - |
dc.contributor.author | Kwon, Taek Hyun | - |
dc.contributor.author | Choi, Hye-Kyoung | - |
dc.contributor.author | Park, Youn Kwan | - |
dc.contributor.author | Kim, Joo Han | - |
dc.date.accessioned | 2021-09-08T01:51:23Z | - |
dc.date.available | 2021-09-08T01:51:23Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2010-07 | - |
dc.identifier.issn | 2005-3711 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/116166 | - |
dc.description.abstract | Objective : Notochordal cells in the intervertebral disc interact with nucleus pulposus (NP) cells and support the maintenance of disc homeostasis by regulation of matrix production. However, the influence of notochordal cells has not been evaluated in the annulus fibrosus (AF), which is the primary pain generator in the disc. We hypothesized that the notochordal cell has the capacity to modulate inflammatory mediators secreted by AF cells secondary to stimulation. Methods : Notochordal and AF cells were isolated from adult New Zealand white rabbits. AF pellets were cultured with notochordal cell clusters or in notochordal cell-conditioned media (NCCM) for 24 or 48 hours with proinflammatory cytokines at varying concentrations. Gene expression in AF pellets were assayed for nitric oxide synthase (iNOS), cyclo-oxygenase (COX)-2, and interleukin (IL)-6 by real time reverse transcriptase polymerase chain reaction (RT-PCR). Results : AF pellet in NCCM significantly decreased the iNOS and COX-2 messenger ribonucleic acid (mRNA) levels compared to AF pellets alone and AF pellets with notochordal cells (p < 0.05). AF pellet resulted in dose-dependent iNOS and COX-2 expression in response to IL-1 beta, stimulation, demonstrating that 1 ng/ml for 24 hours yielded a maximal response. AF pellet in NCCM significantly decreased the expression of iNOS and COX-2 in response to 1ng/ml IL-1 beta, stimulation at 24 hours (p < 0.05). There was no difference in IL-6 expression compared to AF pellets alone or AF pellets with notochordal cell clusters. Conclusion : We conclude that soluble factors from notochordal cells mitigate the gene expression of inflammatory mediators in stimulated AF, as expected after annular injury, suggesting that notochordal cells could serve as a novel therapeutic approach in symptomatic disc development. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN NEUROSURGICAL SOC | - |
dc.subject | INTERVERTEBRAL DISC DEGENERATION | - |
dc.subject | NUCLEUS PULPOSUS CELLS | - |
dc.subject | NECROSIS-FACTOR-ALPHA | - |
dc.subject | LOW-BACK-PAIN | - |
dc.subject | INTERLEUKIN-1-BETA | - |
dc.subject | PROLIFERATION | - |
dc.subject | CHONDROCYTES | - |
dc.subject | CULTURE | - |
dc.title | Notochordal Cells Influence Gene Expression of Inflammatory Mediators of Annulus Fibrosus Cells in Proinflammatory Cytokines Stimulation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kwon, Taek Hyun | - |
dc.contributor.affiliatedAuthor | Park, Youn Kwan | - |
dc.contributor.affiliatedAuthor | Kim, Joo Han | - |
dc.identifier.doi | 10.3340/jkns.2010.48.1.1 | - |
dc.identifier.scopusid | 2-s2.0-77957657981 | - |
dc.identifier.wosid | 000280521400001 | - |
dc.identifier.bibliographicCitation | JOURNAL OF KOREAN NEUROSURGICAL SOCIETY, v.48, no.1, pp.1 - 7 | - |
dc.relation.isPartOf | JOURNAL OF KOREAN NEUROSURGICAL SOCIETY | - |
dc.citation.title | JOURNAL OF KOREAN NEUROSURGICAL SOCIETY | - |
dc.citation.volume | 48 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 7 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001467130 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalResearchArea | Surgery | - |
dc.relation.journalWebOfScienceCategory | Clinical Neurology | - |
dc.relation.journalWebOfScienceCategory | Surgery | - |
dc.subject.keywordPlus | INTERVERTEBRAL DISC DEGENERATION | - |
dc.subject.keywordPlus | NUCLEUS PULPOSUS CELLS | - |
dc.subject.keywordPlus | NECROSIS-FACTOR-ALPHA | - |
dc.subject.keywordPlus | LOW-BACK-PAIN | - |
dc.subject.keywordPlus | INTERLEUKIN-1-BETA | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | CHONDROCYTES | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordAuthor | Notochordal cell | - |
dc.subject.keywordAuthor | Anulus fibrosus | - |
dc.subject.keywordAuthor | Inflammatory mediators | - |
dc.subject.keywordAuthor | Real-time RT-PCR | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.