Albumin expression is required for adipocyte differentiation of 3T3-L1 cells
- Authors
- Yoo, Wonbaek; Lee, Jaeseob; Park, Sangeun; Kim, Young-Sik; Lim, Chaeseung; Yoon, Eulsik; Hur, Gangmin; Oh, Junseo
- Issue Date
- 25-6월-2010
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Albumin; 3T3-L1 adipocyte; Lipid droplet formation; Adipogenesis; PPAR-gamma; C/EBP-alpha
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.397, no.2, pp.170 - 175
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 397
- Number
- 2
- Start Page
- 170
- End Page
- 175
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/116215
- DOI
- 10.1016/j.bbrc.2010.05.067
- ISSN
- 0006-291X
- Abstract
- We have previously demonstrated that albumin is directly involved in the formation of cytoplasmic lipid droplets in pancreatic stellate cells and may act as a downstream effector of adipogenic transcription factors, PPAR-gamma and C/EBP-alpha. Here, we investigated the role of albumin in adipocyte differentiation using 3T3-L1 cells. Albumin expression was significantly increased at later stages of adipocyte differentiation, which was accompanied with increased C/EBP-beta binding to albumin promoter. Suppression of albumin expression using short-hairpin RNA (shRNA) during differentiation led to a considerable reduction in lipid droplet formation, whereas albumin overexpression was stimulatory. Furthermore, point mutation in its fatty acid-binding sites inhibited lipid droplet formation. Consistent with these in vitro finding, Nagase analbuminemic rats displayed reduced fat accumulation. Therefore, our findings suggest that albumin may play a distinct role in adipocyte differentiation by promoting lipid accumulation. (C) 2010 Elsevier Inc. All rights reserved.
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