Ultra-Specific Enrichment of GST-Tagged Protein by GSH-Modified Nanoparticles
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Yeon-Ji | - |
dc.contributor.author | Park, Jong-Moon | - |
dc.contributor.author | Huh, Jiyoung | - |
dc.contributor.author | Kim, Minsik | - |
dc.contributor.author | Lee, Je-Sun | - |
dc.contributor.author | Palani, Arudra | - |
dc.contributor.author | Lee, Kwangyeol | - |
dc.contributor.author | Lee, Sang-Won | - |
dc.date.accessioned | 2021-09-08T02:10:33Z | - |
dc.date.available | 2021-09-08T02:10:33Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2010-06-20 | - |
dc.identifier.issn | 0253-2964 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/116223 | - |
dc.description.abstract | The selective isolation of specific proteins from complex protein mixtures by nanoparticles is reported. Glutathione-modified superparamagnetic nanoparticles were used to purify specific proteins fused with glutathione S-transferase via enzyme-substrate interactions. They demonstrated greatly improved selectivity and efficiency over micron sized capturing beads. The ultra-specific enrichment of target proteins was confirmed by both SDS-PAGE and LC/MS/MS experiments. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.subject | TRANSFERASE FUSION PROTEINS | - |
dc.subject | MASS-SPECTROMETRY | - |
dc.subject | SELECTIVE ENRICHMENT | - |
dc.subject | MAGNETIC NANOPARTICLES | - |
dc.subject | PHOSPHORYLATED PEPTIDES | - |
dc.subject | QUANTIFICATION | - |
dc.subject | IDENTIFICATION | - |
dc.subject | PURIFICATION | - |
dc.subject | PROBE | - |
dc.subject | SIRNA | - |
dc.title | Ultra-Specific Enrichment of GST-Tagged Protein by GSH-Modified Nanoparticles | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Kwangyeol | - |
dc.contributor.affiliatedAuthor | Lee, Sang-Won | - |
dc.identifier.doi | 10.5012/bkcs.2010.31.6.1568 | - |
dc.identifier.scopusid | 2-s2.0-77953936753 | - |
dc.identifier.wosid | 000279126800023 | - |
dc.identifier.bibliographicCitation | BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.31, no.6, pp.1568 - 1572 | - |
dc.relation.isPartOf | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.citation.title | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.citation.volume | 31 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1568 | - |
dc.citation.endPage | 1572 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001525844 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | TRANSFERASE FUSION PROTEINS | - |
dc.subject.keywordPlus | MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | SELECTIVE ENRICHMENT | - |
dc.subject.keywordPlus | MAGNETIC NANOPARTICLES | - |
dc.subject.keywordPlus | PHOSPHORYLATED PEPTIDES | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | PROBE | - |
dc.subject.keywordPlus | SIRNA | - |
dc.subject.keywordAuthor | Proteomics | - |
dc.subject.keywordAuthor | Glutathione | - |
dc.subject.keywordAuthor | Superparamagnetic | - |
dc.subject.keywordAuthor | Nanoparticle | - |
dc.subject.keywordAuthor | Separation | - |
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