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Ultra-Specific Enrichment of GST-Tagged Protein by GSH-Modified Nanoparticles

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dc.contributor.authorLee, Yeon-Ji-
dc.contributor.authorPark, Jong-Moon-
dc.contributor.authorHuh, Jiyoung-
dc.contributor.authorKim, Minsik-
dc.contributor.authorLee, Je-Sun-
dc.contributor.authorPalani, Arudra-
dc.contributor.authorLee, Kwangyeol-
dc.contributor.authorLee, Sang-Won-
dc.date.accessioned2021-09-08T02:10:33Z-
dc.date.available2021-09-08T02:10:33Z-
dc.date.created2021-06-11-
dc.date.issued2010-06-20-
dc.identifier.issn0253-2964-
dc.identifier.urihttps://scholar.korea.ac.kr/handle/2021.sw.korea/116223-
dc.description.abstractThe selective isolation of specific proteins from complex protein mixtures by nanoparticles is reported. Glutathione-modified superparamagnetic nanoparticles were used to purify specific proteins fused with glutathione S-transferase via enzyme-substrate interactions. They demonstrated greatly improved selectivity and efficiency over micron sized capturing beads. The ultra-specific enrichment of target proteins was confirmed by both SDS-PAGE and LC/MS/MS experiments.-
dc.languageEnglish-
dc.language.isoen-
dc.publisherWILEY-V C H VERLAG GMBH-
dc.subjectTRANSFERASE FUSION PROTEINS-
dc.subjectMASS-SPECTROMETRY-
dc.subjectSELECTIVE ENRICHMENT-
dc.subjectMAGNETIC NANOPARTICLES-
dc.subjectPHOSPHORYLATED PEPTIDES-
dc.subjectQUANTIFICATION-
dc.subjectIDENTIFICATION-
dc.subjectPURIFICATION-
dc.subjectPROBE-
dc.subjectSIRNA-
dc.titleUltra-Specific Enrichment of GST-Tagged Protein by GSH-Modified Nanoparticles-
dc.typeArticle-
dc.contributor.affiliatedAuthorLee, Kwangyeol-
dc.contributor.affiliatedAuthorLee, Sang-Won-
dc.identifier.doi10.5012/bkcs.2010.31.6.1568-
dc.identifier.scopusid2-s2.0-77953936753-
dc.identifier.wosid000279126800023-
dc.identifier.bibliographicCitationBULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.31, no.6, pp.1568 - 1572-
dc.relation.isPartOfBULLETIN OF THE KOREAN CHEMICAL SOCIETY-
dc.citation.titleBULLETIN OF THE KOREAN CHEMICAL SOCIETY-
dc.citation.volume31-
dc.citation.number6-
dc.citation.startPage1568-
dc.citation.endPage1572-
dc.type.rimsART-
dc.type.docTypeArticle-
dc.identifier.kciidART001525844-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.description.journalRegisteredClasskci-
dc.relation.journalResearchAreaChemistry-
dc.relation.journalWebOfScienceCategoryChemistry, Multidisciplinary-
dc.subject.keywordPlusTRANSFERASE FUSION PROTEINS-
dc.subject.keywordPlusMASS-SPECTROMETRY-
dc.subject.keywordPlusSELECTIVE ENRICHMENT-
dc.subject.keywordPlusMAGNETIC NANOPARTICLES-
dc.subject.keywordPlusPHOSPHORYLATED PEPTIDES-
dc.subject.keywordPlusQUANTIFICATION-
dc.subject.keywordPlusIDENTIFICATION-
dc.subject.keywordPlusPURIFICATION-
dc.subject.keywordPlusPROBE-
dc.subject.keywordPlusSIRNA-
dc.subject.keywordAuthorProteomics-
dc.subject.keywordAuthorGlutathione-
dc.subject.keywordAuthorSuperparamagnetic-
dc.subject.keywordAuthorNanoparticle-
dc.subject.keywordAuthorSeparation-
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