E2F1-directed activation of Bcl-2 is correlated with lactoferrin-induced apoptosis in Jurkat leukemia T lymphocytes
DC Field | Value | Language |
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dc.contributor.author | Lee, Shin-Hee | - |
dc.contributor.author | Hwang, Hye-Min | - |
dc.contributor.author | Pyo, Chul-Woong | - |
dc.contributor.author | Hahm, Dae Hyun | - |
dc.contributor.author | Choi, Sang-Yun | - |
dc.date.accessioned | 2021-09-08T02:32:57Z | - |
dc.date.available | 2021-09-08T02:32:57Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2010-06 | - |
dc.identifier.issn | 0966-0844 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/116275 | - |
dc.description.abstract | Lactoferrin (Lf) has been shown to control the proliferation of a variety of mammalian cells. Recently, we reported that human Lf induces apoptosis via a c-Jun N-terminal kinases (JNK)-associated Bcl-2 pathway that stimulates programmed cell death. In order to gain insight into the mechanism underlying Lf-triggered apoptotic features, we attempted to determine the mechanisms whereby the Lf-induced Bcl-2 family proteins exert their pro- or anti-apoptotic effects in Jurkat leukemia T lymphocytes. Treatment of the cells with high concentrations of Lf resulted in a significant reduction in in vitro growth and cell viability. As the levels of Lf increased, greater quantities of CDK6 and hyper-phosphorylated retinoblastoma protein were produced, resulting in the induction of E2F1-dependent apoptosis. Simultaneously, PARP and caspases were efficiently cleaved during Lf-induced apoptosis. The E2F1-induced apoptotic process occurred preferentially in p53-deficient Jurkat leukemia cells. Therefore, we attempted to determine whether E2F1-regulated Bcl-2 family proteins involved in the apoptotic process were relevant to Lf-induced apoptosis. We found that Lf increased the interaction of Bcl-2 with the pro-apoptotic protein Bad, whereas the total protein levels did not change significantly. Our results, collectively, suggest that Lf exploits the control mechanism of E2F1-regulated target genes or Bcl-2 family gene networks involved in the apoptotic process in Jurkat human leukemia T lymphocytes. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | CYCLIN-DEPENDENT KINASES | - |
dc.subject | GROWTH ARREST | - |
dc.subject | GENES | - |
dc.subject | E2F1 | - |
dc.subject | DNA | - |
dc.subject | PROTEIN | - |
dc.subject | DAMAGE | - |
dc.subject | CELLS | - |
dc.title | E2F1-directed activation of Bcl-2 is correlated with lactoferrin-induced apoptosis in Jurkat leukemia T lymphocytes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Pyo, Chul-Woong | - |
dc.contributor.affiliatedAuthor | Choi, Sang-Yun | - |
dc.identifier.doi | 10.1007/s10534-010-9341-1 | - |
dc.identifier.wosid | 000277630400017 | - |
dc.identifier.bibliographicCitation | BIOMETALS, v.23, no.3, pp.507 - 514 | - |
dc.relation.isPartOf | BIOMETALS | - |
dc.citation.title | BIOMETALS | - |
dc.citation.volume | 23 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 507 | - |
dc.citation.endPage | 514 | - |
dc.type.rims | ART | - |
dc.type.docType | Article; Proceedings Paper | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | CYCLIN-DEPENDENT KINASES | - |
dc.subject.keywordPlus | GROWTH ARREST | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | E2F1 | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | DAMAGE | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | Lactoferrin | - |
dc.subject.keywordAuthor | Iron | - |
dc.subject.keywordAuthor | Apoptosis Jurkat | - |
dc.subject.keywordAuthor | Bcl-2 | - |
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