Expression, purification and biochemical characterization of the N-terminal regions of human TIG3 and HRASLS3 proteins
DC Field | Value | Language |
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dc.contributor.author | Han, Byeong-Gu | - |
dc.contributor.author | Cho, Jea-Won | - |
dc.contributor.author | Cho, Young-Doo | - |
dc.contributor.author | Kim, Soo-Youl | - |
dc.contributor.author | Yoon, Hye-Jin | - |
dc.contributor.author | Song, Hyun Kyu | - |
dc.contributor.author | Cheong, Hae-Kap | - |
dc.contributor.author | Jeon, Young-Ho | - |
dc.contributor.author | Lee, Dong-Ki | - |
dc.contributor.author | Lee, Sangho | - |
dc.contributor.author | Lee, Byung Il | - |
dc.date.accessioned | 2021-09-08T03:27:04Z | - |
dc.date.available | 2021-09-08T03:27:04Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2010-05 | - |
dc.identifier.issn | 1046-5928 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/116517 | - |
dc.description.abstract | Tarzarotene-induced gene 3 (TIG3) and HRAS-like suppressor (HRASLS3) are members of the HREV107 family of class II tumor suppressors, which are clown-regulated in various cancer cells. TIG3 and HRASLS3 also exhibit phospholipase activities. Both proteins share a common domain architecture with hydrophilic N-terminal and hydrophobic C-terminal regions. The hydrophobic C-terminal region is important for tumor suppression. However, the function of the hydrophilic N-terminal region remains elusive. To facilitate biochemical characterizations of TIG3 and HRASLS3, we expressed and purified the N-terminal regions of TIG3 and HRASLS3, designated TIG3 (1-134) and HRASLS3 (1-133), in a bacterial system. We found that the N-terminal regions of TIG3 and HRASLS3 have calcium-independent phospholipase A(2) activities. Limited proteolysis revealed that TIG3 (1-132) is a structural domain in the N-terminal region of TIG3. Our data suggest that the hydrophobic C-terminal regions might be crucial for Cellular localization, while the hydrophilic N-terminal regions are Sufficient for the enzymatic activity of both TIG3 and HRASLS3. (C) 2010 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | TUMOR-SUPPRESSOR GENE | - |
dc.subject | MITOGEN-ACTIVATED-KINASE | - |
dc.subject | OVARIAN-CARCINOMA CELLS | - |
dc.subject | DOWN-REGULATION | - |
dc.subject | RAS TRAFFICS | - |
dc.subject | CANCER CELLS | - |
dc.subject | H-RAS | - |
dc.subject | PATHWAY | - |
dc.subject | RIG1 | - |
dc.subject | APOPTOSIS | - |
dc.title | Expression, purification and biochemical characterization of the N-terminal regions of human TIG3 and HRASLS3 proteins | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Song, Hyun Kyu | - |
dc.contributor.affiliatedAuthor | Jeon, Young-Ho | - |
dc.identifier.doi | 10.1016/j.pep.2010.01.018 | - |
dc.identifier.scopusid | 2-s2.0-77249111668 | - |
dc.identifier.wosid | 000275485900016 | - |
dc.identifier.bibliographicCitation | PROTEIN EXPRESSION AND PURIFICATION, v.71, no.1, pp.103 - 107 | - |
dc.relation.isPartOf | PROTEIN EXPRESSION AND PURIFICATION | - |
dc.citation.title | PROTEIN EXPRESSION AND PURIFICATION | - |
dc.citation.volume | 71 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 103 | - |
dc.citation.endPage | 107 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | TUMOR-SUPPRESSOR GENE | - |
dc.subject.keywordPlus | MITOGEN-ACTIVATED-KINASE | - |
dc.subject.keywordPlus | OVARIAN-CARCINOMA CELLS | - |
dc.subject.keywordPlus | DOWN-REGULATION | - |
dc.subject.keywordPlus | RAS TRAFFICS | - |
dc.subject.keywordPlus | CANCER CELLS | - |
dc.subject.keywordPlus | H-RAS | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | RIG1 | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordAuthor | TIG3 | - |
dc.subject.keywordAuthor | RIG1 | - |
dc.subject.keywordAuthor | RARRES3 | - |
dc.subject.keywordAuthor | HRASLS3 | - |
dc.subject.keywordAuthor | HREV107 | - |
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