Identification of toxicological biomarkers of di(2-ethylhexyl) phthalate in proteins secreted by HepG2 cells using proteomic analysis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Seonyoung | - |
dc.contributor.author | Park, So-Young | - |
dc.contributor.author | Jeong, Ji | - |
dc.contributor.author | Cho, Eunkyung | - |
dc.contributor.author | Phark, Sohee | - |
dc.contributor.author | Lee, Min | - |
dc.contributor.author | Kwak, Dongsub | - |
dc.contributor.author | Lim, Ji-Youn | - |
dc.contributor.author | Jung, Woon-Won | - |
dc.contributor.author | Sul, Donggeun | - |
dc.date.accessioned | 2021-09-08T03:31:01Z | - |
dc.date.available | 2021-09-08T03:31:01Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2010-05 | - |
dc.identifier.issn | 1615-9853 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/116537 | - |
dc.description.abstract | The effects of di(2-ethylhexyl) phthalate (DEHP) on proteins secreted by HepG2 cells were studied using a proteomic approach. HepG2 cells were exposed to various concentrations of DEHP (0, 2.5, 5, 10, 25, 50, 100, and 250 mu M) for 24 or 48h. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and comet assays were then conducted to determine the cytotoxicity and genotoxicity of DEHP, respectively. The MIT assay showed that 10 mu M DEHP was the maximum concentration that did not cause cell death. In addition, the DNA damage in HepG2 cells exposed to DEHP was found to increase in a dose- and time-dependent fashion. Proteomic analysis using two different pI ranges (4-7 and 6-9) and large size 2-DE revealed the presence of 2776 protein spots. A total of 35 (19 up- and 16 down-regulated) proteins were identified as biomarkers of DEHP by ESI-MS/MS. Several differentiated protein groups were also found. Proteins involved in apoptosis, transportation, signaling, energy metabolism, and cell structure and motility were found to be up- or down-regulated. Among these, the identities of cystatin C, Rho GDP inhibitor, retinol binding protein 4, gelsolin, DEK protein, Raf kinase inhibitory protein, triose phosphate isomerase, cofilin-1, and haptoglobin-related protein were confirmed by Western blot assay. Therefore, these proteins could be used as potential biomarkers of DEHP and human disease associated with DEHP. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | CHEMICALLY-INDUCED CHANGES | - |
dc.subject | BINDING-PROTEIN | - |
dc.subject | CYSTATIN-C | - |
dc.subject | PLASMA-PROTEINS | - |
dc.subject | DI-(2-ETHYLHEXYL) PHTHALATE | - |
dc.subject | PEROXISOME PROLIFERATION | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | HUMAN SPERM | - |
dc.subject | DNA-DAMAGE | - |
dc.subject | EXPRESSION | - |
dc.title | Identification of toxicological biomarkers of di(2-ethylhexyl) phthalate in proteins secreted by HepG2 cells using proteomic analysis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Phark, Sohee | - |
dc.contributor.affiliatedAuthor | Jung, Woon-Won | - |
dc.contributor.affiliatedAuthor | Sul, Donggeun | - |
dc.identifier.doi | 10.1002/pmic.200900674 | - |
dc.identifier.scopusid | 2-s2.0-77956396298 | - |
dc.identifier.wosid | 000277730900009 | - |
dc.identifier.bibliographicCitation | PROTEOMICS, v.10, no.9, pp.1831 - 1846 | - |
dc.relation.isPartOf | PROTEOMICS | - |
dc.citation.title | PROTEOMICS | - |
dc.citation.volume | 10 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1831 | - |
dc.citation.endPage | 1846 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | CHEMICALLY-INDUCED CHANGES | - |
dc.subject.keywordPlus | BINDING-PROTEIN | - |
dc.subject.keywordPlus | CYSTATIN-C | - |
dc.subject.keywordPlus | PLASMA-PROTEINS | - |
dc.subject.keywordPlus | DI-(2-ETHYLHEXYL) PHTHALATE | - |
dc.subject.keywordPlus | PEROXISOME PROLIFERATION | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | HUMAN SPERM | - |
dc.subject.keywordPlus | DNA-DAMAGE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | Animal proteomics | - |
dc.subject.keywordAuthor | Biomarkers | - |
dc.subject.keywordAuthor | Di(2-ethylhexyl) phthalate | - |
dc.subject.keywordAuthor | HepG2 | - |
dc.subject.keywordAuthor | Secreted proteins | - |
dc.subject.keywordAuthor | Two-dimensional polyacrylamide gel electrophoresis | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.