Flavobacterium fluvii sp nov., isolated from stream sediment

Abstract

An aerobic, yellow-pigmented, Gram-staining-negative bacterium, designated strain H7(T), was isolated from sediment of Gazwa stream in Jinju city, South Korea. Cells of strain H7(T) were nonmotile, straight rods that produced flexirubin pigments and showed catalase- and oxidase activities. Strain H7(T) contained iso-C-15:0, anteiso-C-15:0 and summed feature 3 (comprising C-16:1 omega 7c and/or iso-C-15:0 2-OH) as the main fatty acids and menaquinone-6 (MK-6) as the major isoprenoid quinone. The DNA G+C content of strain H7(T) was 37.2 mol%. Comparative 16S rRNA gene sequence analysis showed that strain H7(T) formed a distinct phyletic line within the genus Flavobacterium. Based on 16S rRNA gene sequence similarities, the novel strain was most closely related to Flavobacterium, limicola ST-82(T) and Flavobacterium, resistens BD-b365(T) with 97.2% and 97.0% gene sequence similarities, respectively. The DNA-DNA relatedness of the novel strain with these species was only 18% and 14%, respectively. On the basis of phenotypic data and molecular properties, strain H7(T) represents a novel species within the genus Flavobacterium, for which the name Flavobacterium fluvii sp. nov. is proposed. The type strain is H7(T) (=KACC 12818(T) =DSM 19978(T)).