Nanobiocatalysis for protein digestion in proteomic analysis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jungbae | - |
dc.contributor.author | Kim, Byoung Chan | - |
dc.contributor.author | Lopez-Ferrer, Daniel | - |
dc.contributor.author | Petritis, Konstantinos | - |
dc.contributor.author | Smith, Richard D. | - |
dc.date.accessioned | 2021-09-08T05:31:54Z | - |
dc.date.available | 2021-09-08T05:31:54Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2010-02 | - |
dc.identifier.issn | 1615-9853 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/117088 | - |
dc.description.abstract | The process of protein digestion is a critical step for successful protein identification in bottom-up proteomic analyses. To substitute the present practice of in-solution protein digestion, which is long, tedious, and difficult to automate, many efforts have been dedicated for the development of a rapid, recyclable and automated digestion system. Recent advances of nanobiocatalytic approaches have improved the performance of protein digestion by using various nanomaterials such as nanoporous materials, magnetic nanoparticles, and polymer nanofibers. Especially, the unprecedented success of trypsin stabilization in the form of trypsin-coated nanofibers, showing no activity decrease under repeated uses for 1 year and retaining good resistance to proteolysis, has demonstrated its great potential to be employed in the development of automated, high-throughput, and on-line digestion systems. This review discusses recent developments of nanobiocatalytic approaches for the improved performance of protein digestion in speed, detection sensitivity, recyclability, and trypsin stability. In addition, we also introduce approaches for protein digestion under unconventional energy input for protein denaturation and the development of microfluidic enzyme reactors that can benefit from recent successes of these nanobiocatalytic approaches. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | INTENSITY FOCUSED ULTRASOUND | - |
dc.subject | HIGHLY EFFICIENT PROTEOLYSIS | - |
dc.subject | DESORPTION/IONIZATION MASS-SPECTROMETRY | - |
dc.subject | IMMOBILIZED MAGNETIC NANOPARTICLES | - |
dc.subject | MONOLITHIC ENZYMATIC MICROREACTOR | - |
dc.subject | LESS COMMON APPLICATIONS | - |
dc.subject | TOF-MS ANALYSIS | - |
dc.subject | ON-A-CHIP | - |
dc.subject | LIQUID-CHROMATOGRAPHY | - |
dc.subject | POLYACRYLAMIDE-GELS | - |
dc.title | Nanobiocatalysis for protein digestion in proteomic analysis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jungbae | - |
dc.identifier.doi | 10.1002/pmic.200900519 | - |
dc.identifier.scopusid | 2-s2.0-77149121079 | - |
dc.identifier.wosid | 000275260700008 | - |
dc.identifier.bibliographicCitation | PROTEOMICS, v.10, no.4, pp.687 - 699 | - |
dc.relation.isPartOf | PROTEOMICS | - |
dc.citation.title | PROTEOMICS | - |
dc.citation.volume | 10 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 687 | - |
dc.citation.endPage | 699 | - |
dc.type.rims | ART | - |
dc.type.docType | Review | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | INTENSITY FOCUSED ULTRASOUND | - |
dc.subject.keywordPlus | HIGHLY EFFICIENT PROTEOLYSIS | - |
dc.subject.keywordPlus | DESORPTION/IONIZATION MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | IMMOBILIZED MAGNETIC NANOPARTICLES | - |
dc.subject.keywordPlus | MONOLITHIC ENZYMATIC MICROREACTOR | - |
dc.subject.keywordPlus | LESS COMMON APPLICATIONS | - |
dc.subject.keywordPlus | TOF-MS ANALYSIS | - |
dc.subject.keywordPlus | ON-A-CHIP | - |
dc.subject.keywordPlus | LIQUID-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | POLYACRYLAMIDE-GELS | - |
dc.subject.keywordAuthor | Enzyme coating | - |
dc.subject.keywordAuthor | Nanobiocatalysis | - |
dc.subject.keywordAuthor | Nanoproteomics | - |
dc.subject.keywordAuthor | Protein digestion | - |
dc.subject.keywordAuthor | Trypsin stabilization | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.