Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Immunogold-silver staining-on-a-chip biosensor based on cross-flow chromatography's

Authors
Cho, Il-HoonSeo, Sung-MinPaek, Eui-HwanPaek, Se-Hwan
Issue Date
15-1월-2010
Publisher
ELSEVIER SCIENCE BV
Keywords
Silver intensification; Non-laboratory immunoassay; Background staining control; Self-nucleation ions; Detection capability; Cardiac troponin I
Citation
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.878, no.2, pp.271 - 277
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume
878
Number
2
Start Page
271
End Page
277
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/117160
DOI
10.1016/j.jchromb.2009.07.016
ISSN
1570-0232
Abstract
Immunogold-silver staining (IGSS) was adopted in cross-flow chromatographic analysis in which immunological reactions and silver intensification were sequentially conducted in the vertical and horizontal directions, respectively Factors controlling the performance. except the silver substrate solution. were optimized to increase the signal-to-background ratio in measurements of cardiac troponin I as a model analyte In generating the signal, the size of colloidal gold catalyst was critical. the smallest size (5-nm diameter) in the selected range yielded the highest colorimeric signal. To maintain the low background, two processes, blocking the remaining surfaces of membrane after antibody immobilization and washing the residual tracer after immunological reaction, were necessary. Self-nucleation of silver ions also caused a background signal and was controlled to some degree by decreasing the hydrodynamic force that arose when the substrate solution was supplied in the horizontal direction finally, a new chip (IGSS-on-a-chip, IOC) that allowed for convenient, efficient IGSS was produced by injection molding of plastic This method enhanced the detection capability by 51-fold compared to the conventional rapid test kit using 30 nm-sized colloidal gold as the tracer. The IOC biosensor results also showed that silver intensification yield via cross flow after Immunological reaction was 19% higher than that by traditional incubation. (C) 2009 Elsevier B.V All rights reserved
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Science and Technology > Department of Biotechnology and Bioinformatics > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE