MODIFIED VACUOLE PHENOTYPE1 Is an Arabidopsis Myrosinase-Associated Protein Involved in Endomembrane Protein Trafficking
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Agee, April E. | - |
dc.contributor.author | Surpin, Marci | - |
dc.contributor.author | Sohn, Eun Ju | - |
dc.contributor.author | Girke, Thomas | - |
dc.contributor.author | Rosado, Abel | - |
dc.contributor.author | Kram, Brian W. | - |
dc.contributor.author | Carter, Clay | - |
dc.contributor.author | Wentzell, Adam M. | - |
dc.contributor.author | Kliebenstein, Daniel J. | - |
dc.contributor.author | Jin, Hak Chul | - |
dc.contributor.author | Park, Ohkmae K. | - |
dc.contributor.author | Jin, Hailing | - |
dc.contributor.author | Hicks, Glenn R. | - |
dc.contributor.author | Raikhel, Natasha V. | - |
dc.date.accessioned | 2021-09-08T06:14:39Z | - |
dc.date.available | 2021-09-08T06:14:39Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2010-01 | - |
dc.identifier.issn | 0032-0889 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/117306 | - |
dc.description.abstract | We identified an Arabidopsis (Arabidopsis thaliana) ethyl methanesulfonate mutant, modified vacuole phenotype1-1 (mvp1-1), in a fluorescent confocal microscopy screen for plants with mislocalization of a green fluorescent protein-delta tonoplast intrinsic protein fusion. The mvp1-1 mutant displayed static perinuclear aggregates of the reporter protein. mvp1 mutants also exhibited a number of vacuole-related phenotypes, as demonstrated by defects in growth, utilization of stored carbon, gravitropic response, salt sensitivity, and specific susceptibility to the fungal necrotroph Alternaria brassicicola. Similarly, crosses with other endomembrane marker fusions identified mislocalization to aggregate structures, indicating a general defect in protein trafficking. Map-based cloning showed that the mvp1-1 mutation altered a gene encoding a putative myrosinase-associated protein, and glutathione S-transferase pull-down assays demonstrated that MVP1 interacted specifically with the Arabidopsis myrosinase protein, THIOGLUCOSIDE GLUCOHYDROLASE2 (TGG2), but not TGG1. Moreover, the mvp1-1 mutant showed increased nitrile production during glucosinolate hydrolysis, suggesting that MVP1 may play a role in modulation of myrosinase activity. We propose that MVP1 is a myrosinase-associated protein that functions, in part, to correctly localize the myrosinase TGG2 and prevent inappropriate glucosinolate hydrolysis that could generate cytotoxic molecules. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC PLANT BIOLOGISTS | - |
dc.subject | GLUCOSINOLATE HYDROLYSIS | - |
dc.subject | SHOOT GRAVITROPISM | - |
dc.subject | METHYL JASMONATE | - |
dc.subject | BETA-GLUCOSIDASE | - |
dc.subject | PLANT-CELLS | - |
dc.subject | ER BODY | - |
dc.subject | THALIANA | - |
dc.subject | GENE | - |
dc.subject | DEFENSE | - |
dc.subject | CLONING | - |
dc.title | MODIFIED VACUOLE PHENOTYPE1 Is an Arabidopsis Myrosinase-Associated Protein Involved in Endomembrane Protein Trafficking | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Ohkmae K. | - |
dc.identifier.doi | 10.1104/pp.109.145078 | - |
dc.identifier.scopusid | 2-s2.0-73249121280 | - |
dc.identifier.wosid | 000273148100010 | - |
dc.identifier.bibliographicCitation | PLANT PHYSIOLOGY, v.152, no.1, pp.120 - 132 | - |
dc.relation.isPartOf | PLANT PHYSIOLOGY | - |
dc.citation.title | PLANT PHYSIOLOGY | - |
dc.citation.volume | 152 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 120 | - |
dc.citation.endPage | 132 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.subject.keywordPlus | GLUCOSINOLATE HYDROLYSIS | - |
dc.subject.keywordPlus | SHOOT GRAVITROPISM | - |
dc.subject.keywordPlus | METHYL JASMONATE | - |
dc.subject.keywordPlus | BETA-GLUCOSIDASE | - |
dc.subject.keywordPlus | PLANT-CELLS | - |
dc.subject.keywordPlus | ER BODY | - |
dc.subject.keywordPlus | THALIANA | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | DEFENSE | - |
dc.subject.keywordPlus | CLONING | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.