세포 호흡량 측정용 다채널 측정 시스템 개발

Abstract

This paper describes a multi channel measurement system which can measure the cellular respiration level in a solution containing cells by using a Clark-type sensor with the solution temperature control unit. The Clark-type sensor can measure the cellular respiration level in the solution because it can measure the reduction current depending on the dissolved oxygen level in the solution. This measurement system was maintained the temperature within ± 0.1 oC of the setting temperature value by on/off control method in order to measure the precise cellular respiration level. The measurement system showed that the applied voltage to the working electrode was very stable(−0.8 V± 0.0071 V) by using proportional control method. From the current measurement, the response time and the linearity correlation coefficient were 25 sec and 0.94, respectively, which are very close to the results of the commercial product. Using this system and the fabricated Clarktype sensor, the average ratio of the uncoupled OCR(oxygen consumption rate) to the coupled OCR was 1.35 and this is almost the same as that obtained from a commercial systems.