감마선 처리에 의한 에스트로겐 활성 저감 연구

Abstract

In this study, degradation of estrone (E1) and 17α-ethynylestradiol (EE2) by gamma-irradiation and subsequent reduction of estrogenic activity as a function of absorbed dose were conducted using the yeast two-hybrid assay. Relative potency of E1 and EE2 compared to estrogenic activity of 17β-estradiol (E2) was found to be 0.0144 and 0.1605, respectively. More than 90% of E1 and EE2 (both 5.0 × 10-6 M) was removed at an absorbed dose of 5 kGy, but more than 40% of estrogenic activity still remained. The addition of TiO2 catalyst appeared to improve the removal efficiency of E1 and decrease estrogenic activity while there was no significant effect for EE2. Additionally, the calculated estrogenic activity of E1 and EE2 based on a regression model was well correlated with the observed activity.