SIMPLE DETERMINATION OF ERDOSTEINE IN HUMAN PLASMA USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
- Authors
- Kim, Sung Tae; Park, Jeong-Sook; Kim, Hyung Tae; Kim, Chong-Kook
- Issue Date
- 2010
- Publisher
- TAYLOR & FRANCIS INC
- Keywords
- bioequivalence; erdosteine; HPLC; UV
- Citation
- JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, v.33, no.13, pp.1319 - 1327
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
- Volume
- 33
- Number
- 13
- Start Page
- 1319
- End Page
- 1327
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/118672
- DOI
- 10.1080/10826076.2010.489019
- ISSN
- 1082-6076
- Abstract
- A simple and rapid high-performance liquid chromatography (HPLC) method with UV detection (220nm) was developed for the determination of erdosteine in human plasma. The plasma was prepared by deproteination based on treatment with 6.25% trichloroacetic acid (TCA). After the mixture was centrifuged, the supernatant was separated on CAPCELL PACK C18(4.6x250mm) column. The mobile phase consisted of acetonitrile in phosphate-heptane sulfonate buffer at the volume ration of 5:95 (pH 2.0). Citiolone was used as an internal standard. The calibration curve was linear in concentrations of 0.5-8g/mL with correlation coefficient of 0.999. The limit of quantitation (LOQ) was 0.5g/mL. This method was sensitive with reproducibility and specificity and successfully applied to the bioequivalence study of erdosteine (900mg) in healthy subjects.
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