SIMPLE DETERMINATION OF ERDOSTEINE IN HUMAN PLASMA USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

A simple and rapid high-performance liquid chromatography (HPLC) method with UV detection (220nm) was developed for the determination of erdosteine in human plasma. The plasma was prepared by deproteination based on treatment with 6.25% trichloroacetic acid (TCA). After the mixture was centrifuged, the supernatant was separated on CAPCELL PACK C18(4.6x250mm) column. The mobile phase consisted of acetonitrile in phosphate-heptane sulfonate buffer at the volume ration of 5:95 (pH 2.0). Citiolone was used as an internal standard. The calibration curve was linear in concentrations of 0.5-8g/mL with correlation coefficient of 0.999. The limit of quantitation (LOQ) was 0.5g/mL. This method was sensitive with reproducibility and specificity and successfully applied to the bioequivalence study of erdosteine (900mg) in healthy subjects.