Effects of Polycaprolactone-Tricalcium Phosphate, Recombinant Human Bone Morphogenetic Protein-2 and Dog Mesenchymal Stem Cells on Bone Formation: Pilot Study in Dogs
DC Field | Value | Language |
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dc.contributor.author | Kim, Sun-Jong | - |
dc.contributor.author | Kim, Myung-Rae | - |
dc.contributor.author | Oh, Jin-Sub | - |
dc.contributor.author | Han, Inho | - |
dc.contributor.author | Shin, Sang-Wan | - |
dc.date.accessioned | 2021-09-08T10:35:51Z | - |
dc.date.available | 2021-09-08T10:35:51Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2009-12-31 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/118722 | - |
dc.description.abstract | Purpose: The aim of this study was to evaluate the survival, proliferation, and bone formation of dog mesenchymal stem cells (dMSCs) in the graft material by using Polycaprolactone-tricalcium phosphate (PCL-TCP), auto-fibrin glue (AFG), recombinant human bone morphogenetic protein-2 (rhBMP-2), and dMSCs after a transplantation to the scapula of adult beagle dogs. Materials and Methods: The subjects were two beagle dogs. Total dose of rhBMP-2 on each block was 10 mu g with 50 mu g/mg concentration. The cortical bone of the scapula of the dog was removed which was the same size of PCL-TCP block (Osteopore International Pte, Singapore; 5.0 x 5.0 x 8.0 mm in size), and the following graft material then was fixed with orthodontic mini-implant, Dual-top (R) (Titanium alloy, Jeil Co. Seoul, Korea). Four experimental groups were prepared for this study, Group 1: PCL-TCP + aFG; Group 2: PCL-TCP + aFG + dMSCs; Group 3: PCL-TCP + aFG + dMSCs, + rhBMP-2; Group 4: PCL-TCP + aFG + dMSCs + rhBMP-2 + PCL membrane. The survival or proliferation of dMSCs cells was identified with an extracted tissue through a fluorescence microscope, H-E staining and Von-Kossa staining in two weeks and four weeks after the transplantation. Results: The survival and proliferation of dMSCs were identified through a fluorescence microscope from both Group 1 and Group 2 in two weeks and four weeks after the transplantation. Histological observation also found that the injected cells were proliferating well in the G2, G3, and G4 scaffolds. Conclusion: This study concluded that bone ingrowth occurred in PCL-TCP scaffold which was transplanted with rhBMP-2, and MSCs did not affect bone growth. More sufficient healing time would be needed to recognize effects of dMSCs on bone formation. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | YONSEI UNIV COLL MEDICINE | - |
dc.subject | AUTOLOGOUS FIBRIN GLUE | - |
dc.subject | TISSUE-ENGINEERED BONE | - |
dc.subject | DELIVERY-SYSTEMS | - |
dc.subject | SCAFFOLDS | - |
dc.subject | REGENERATION | - |
dc.subject | DEGRADATION | - |
dc.subject | RHBMP-2 | - |
dc.title | Effects of Polycaprolactone-Tricalcium Phosphate, Recombinant Human Bone Morphogenetic Protein-2 and Dog Mesenchymal Stem Cells on Bone Formation: Pilot Study in Dogs | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, Sang-Wan | - |
dc.identifier.doi | 10.3349/ymj.2009.50.6.825 | - |
dc.identifier.scopusid | 2-s2.0-74549177537 | - |
dc.identifier.wosid | 000272993700015 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, v.50, no.6, pp.825 - 831 | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.citation.title | YONSEI MEDICAL JOURNAL | - |
dc.citation.volume | 50 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 825 | - |
dc.citation.endPage | 831 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001397531 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | General & Internal Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, General & Internal | - |
dc.subject.keywordPlus | AUTOLOGOUS FIBRIN GLUE | - |
dc.subject.keywordPlus | TISSUE-ENGINEERED BONE | - |
dc.subject.keywordPlus | DELIVERY-SYSTEMS | - |
dc.subject.keywordPlus | SCAFFOLDS | - |
dc.subject.keywordPlus | REGENERATION | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | RHBMP-2 | - |
dc.subject.keywordAuthor | PCL-TCP scaffold | - |
dc.subject.keywordAuthor | dog MSCs | - |
dc.subject.keywordAuthor | recombinant human bone morphogenic protein-2 | - |
dc.subject.keywordAuthor | auto-fibrin glue | - |
dc.subject.keywordAuthor | bone formation | - |
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