An ELISA-on-a-Chip Biosensor System for Early Screening of Listeria monocytogenes in Contaminated Food Products
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Seo, Sung-Min | - |
dc.contributor.author | Cho, Il-Hoon | - |
dc.contributor.author | Kim, Joo-Ho | - |
dc.contributor.author | Jeon, Jin-Woo | - |
dc.contributor.author | Oh, Eun-Gyoung | - |
dc.contributor.author | Yu, Hong-Sik | - |
dc.contributor.author | Shin, Soon-Bum | - |
dc.contributor.author | Lee, Hee-Jung | - |
dc.contributor.author | Paek, Se-Hwan | - |
dc.date.accessioned | 2021-09-08T10:37:16Z | - |
dc.date.available | 2021-09-08T10:37:16Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2009-12-20 | - |
dc.identifier.issn | 0253-2964 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/118730 | - |
dc.description.abstract | An enzyme-linked immunosorbent assay (ELISA)-on-a-chip (EOC) biosensor combined with cell concentration technology based on immuno-magnetic separation (IMS) was investigated for use as a potential tool for early screening of Listeria monocytogenes (L. monocytogenes) in food products. The target analyte is a well-known pathogenic food-borne microorganism and outbreaks of the food poisoning typically occur due to contamination of normal food products. Thus, the aim of this study was to develop a rapid and reliable sensor that could be utilized on a daily basis to test food products for the presence of this pathogenic microorganism. The sensor was optimized to provide a high detection capability (e.g., 5.9 x 10(3) cells/mL) and, to eventually minimize cultivation time. The cell density was condensed using IMS prior to analysis. Since the concentration rate of was greater than 100-fold, this combination resulted in a detection limit 0154 cells/mL. The EOC-IMS coupled analytical system was then applied to a real sample test of fish intestines. The system was able to detect L. monocytogenes at a concentration of 2.4 CFU/g after pre-enrichment for 6 h from the onset of cell cultivation. This may allow us to monitor the target analyte at a concentration less than I CFU/g within a 9 h-cultivation provided a doubling time of 40 min is typically maintained. Based on this estimation, the EOC-IMS system can screen and detect the presence of this microorganism in food products almost within working hours. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.subject | MONOCLONAL-ANTIBODY | - |
dc.subject | INOCULUM SIZE | - |
dc.subject | STRIP TEST | - |
dc.subject | IMMUNOCHROMATOGRAPHY | - |
dc.subject | MEDIA | - |
dc.subject | PCR | - |
dc.subject | ENRICHMENT | - |
dc.subject | SEPARATION | - |
dc.subject | GROWTH | - |
dc.subject | ASSAY | - |
dc.title | An ELISA-on-a-Chip Biosensor System for Early Screening of Listeria monocytogenes in Contaminated Food Products | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Paek, Se-Hwan | - |
dc.identifier.doi | 10.5012/bkcs.2009.30.12.2993 | - |
dc.identifier.scopusid | 2-s2.0-76649099174 | - |
dc.identifier.wosid | 000276083700034 | - |
dc.identifier.bibliographicCitation | BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.30, no.12, pp.2993 - 2998 | - |
dc.relation.isPartOf | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.citation.title | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.citation.volume | 30 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 2993 | - |
dc.citation.endPage | 2998 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001525140 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | MONOCLONAL-ANTIBODY | - |
dc.subject.keywordPlus | INOCULUM SIZE | - |
dc.subject.keywordPlus | STRIP TEST | - |
dc.subject.keywordPlus | IMMUNOCHROMATOGRAPHY | - |
dc.subject.keywordPlus | MEDIA | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordPlus | ENRICHMENT | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordAuthor | Food-borne microorganism | - |
dc.subject.keywordAuthor | Early detection | - |
dc.subject.keywordAuthor | Quantitative analysis | - |
dc.subject.keywordAuthor | High sensitivity | - |
dc.subject.keywordAuthor | Immunomagnetic separation | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.