Quantitative Imaging of Molecular Order in Lipid Membranes Using Two-Photon Fluorescence Polarimetry
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gasecka, Alicja | - |
dc.contributor.author | Han, Tsai-Jung | - |
dc.contributor.author | Favard, Cyril | - |
dc.contributor.author | Cho, Bong Rae | - |
dc.contributor.author | Brasselet, Sophie | - |
dc.date.accessioned | 2021-09-08T11:30:12Z | - |
dc.date.available | 2021-09-08T11:30:12Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2009-11-18 | - |
dc.identifier.issn | 0006-3495 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/118913 | - |
dc.description.abstract | We present a polarimetric two-photon microscopy technique to quantitatively image the local static molecular orientational behavior in lipid and cell membranes. This approach, based on a tunable excitation polarization state complemented by a polarized readout, is easily implementable and does not require hypotheses on the molecular angular distribution such as its mean orientation, which is a main limitation in traditional fluorescence anisotropy measurements. The method is applied to the investigation of the molecular angular distribution in giant unilamellar vesicles formed by liquid-ordered and liquid-disordered micro-domains, and in COS-7 cell membranes. The highest order contrast between ordered and disordered domains is obtained for dyes locating within the membrane acyl chains. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | CELL PRESS | - |
dc.subject | MODEL MEMBRANES | - |
dc.subject | ANISOTROPY MEASUREMENTS | - |
dc.subject | LINEAR DICHROISM | - |
dc.subject | CELL | - |
dc.subject | ORIENTATION | - |
dc.subject | RAFTS | - |
dc.subject | DOMAINS | - |
dc.subject | PHASE | - |
dc.subject | PROBE | - |
dc.subject | TRANSITION | - |
dc.title | Quantitative Imaging of Molecular Order in Lipid Membranes Using Two-Photon Fluorescence Polarimetry | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Cho, Bong Rae | - |
dc.identifier.doi | 10.1016/j.bpj.2009.08.052 | - |
dc.identifier.scopusid | 2-s2.0-72149121153 | - |
dc.identifier.wosid | 000271917700025 | - |
dc.identifier.bibliographicCitation | BIOPHYSICAL JOURNAL, v.97, no.10, pp.2854 - 2862 | - |
dc.relation.isPartOf | BIOPHYSICAL JOURNAL | - |
dc.citation.title | BIOPHYSICAL JOURNAL | - |
dc.citation.volume | 97 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 2854 | - |
dc.citation.endPage | 2862 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | MODEL MEMBRANES | - |
dc.subject.keywordPlus | ANISOTROPY MEASUREMENTS | - |
dc.subject.keywordPlus | LINEAR DICHROISM | - |
dc.subject.keywordPlus | CELL | - |
dc.subject.keywordPlus | ORIENTATION | - |
dc.subject.keywordPlus | RAFTS | - |
dc.subject.keywordPlus | DOMAINS | - |
dc.subject.keywordPlus | PHASE | - |
dc.subject.keywordPlus | PROBE | - |
dc.subject.keywordPlus | TRANSITION | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.