Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

A Truncated Form of p23 Down-regulates Telomerase Activity via Disruption of Hsp90 Function

Authors
Woo, Sang HyeokAn, SungkwanLee, Hyung-ChahnJin, Hyeon-OkSeo, Sung-KeumYoo, Doo-HyunLee, Kee-HoRhee, Chang HunChoi, Eui-JuHong, Seok-IlPark, In-Chul
Issue Date
6-11월-2009
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Citation
JOURNAL OF BIOLOGICAL CHEMISTRY, v.284, no.45, pp.30871 - 30880
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume
284
Number
45
Start Page
30871
End Page
30880
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/118933
DOI
10.1074/jbc.M109.052720
ISSN
0021-9258
Abstract
The Hsp90-associated protein p23 modulates Hsp90 activity during the final stages of the chaperone pathway to facilitate maturation of client proteins. Previous reports indicate that p23 cleavage induced by caspases during cell death triggers destabilization of client proteins. However, the specific role of truncated p23 (Delta p23) in this process and the underlying mechanisms remain to be determined. One such client protein, hTERT, is a telomerase catalytic subunit regulated by several chaperone proteins, including Hsp90 and p23. In the present study, we examined the effects of p23 cleavage on hTERT stability and telomerase activity. Our data showed that overexpression of Delta p23 resulted in a decrease in hTERT levels, and a down-regulation in telomerase activity. Serine phosphorylation of Hsp90 was significantly reduced in cells expressing high levels of Delta p23 compared with those expressing full-length p23. Mutation analyses revealed that two serine residues (Ser-231 and Ser-263) in Hsp90 are important for activation of telomerase, and down-regulation of telomerase activity by Delta p23 was associated with inhibition of cell growth and sensitization of cells to cisplatin. Our data aid in determining the mechanism underlying the regulation of telomerase activity by the chaperone complex during caspase-dependent cell death.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Life Sciences > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE