Nonstructural 5A protein activates beta-catenin signaling cascades: Implication of hepatitis C virus-induced liver pathogenesis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Chul-Yong | - |
dc.contributor.author | Choi, Soo-Ho | - |
dc.contributor.author | Kang, Sang-Min | - |
dc.contributor.author | Kang, Ju-Il | - |
dc.contributor.author | Ahn, Byung-Yoon | - |
dc.contributor.author | Kim, Hoguen | - |
dc.contributor.author | Jung, Guhung | - |
dc.contributor.author | Choi, Kang-Yell | - |
dc.contributor.author | Hwang, Soon B. | - |
dc.date.accessioned | 2021-09-08T11:51:28Z | - |
dc.date.available | 2021-09-08T11:51:28Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2009-11 | - |
dc.identifier.issn | 0168-8278 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/118956 | - |
dc.description.abstract | Background/Aims: The nonstructural 5A (NS5A) protein of hepatitis C virus (HCV) has been implicated in HCV-induced liver pathogenesis. Wnt/beta-catenin signaling has also been involved in tumorigenesis. To elucidate the molecular mechanism of HCV pathogenesis, we examined the potential effects of HCV NS5A protein on Wnt/beta-catenin signal transduction cascades. Methods:The effects of NS5A protein on beta-catenin signaling cascades in hepatic cells were investigated by luciferase reporter gene assay, confocal microscopy, immunoprecipitation assay, and immunoblot analysis. Results: beta-Catenin-mediated transcriptional activity is elevated by NS5A protein, in the context of HCV replication, and by infection of cell culture-produced HCV. NS5A protein directly interacts with endogenous beta-catenin and colocalizes with beta-catenin in the cytoplasm. NS5A protein inactivates glycogen synthase kinase 3 beta and increases subsequent accumulation of beta-catenin in HepG2 cells. beta-Catenin was also accumulated in HCV patients' liver tissues. In addition, the accumulation of beta-catenin in HCV replicon cells requires both activation of phosphatidylinositol 3-kinase and inactivation of GSK3 beta. Conclusions: NS5A activates beta-catenin signaling cascades through increasing the stability of beta-catenin. This modulation is accomplished by the protein interplay between viral and cellular signaling transducer. These data suggest that NS5A protein may directly be involved in Wnt/beta-catenin-mediated liver pathogenesis. (C) 2009 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | HUMAN HEPATOCELLULAR CARCINOMAS | - |
dc.subject | GLYCOGEN-SYNTHASE KINASE-3 | - |
dc.subject | NS5A PROTEIN | - |
dc.subject | RNA REPLICATION | - |
dc.subject | CELLS | - |
dc.subject | CANCER | - |
dc.subject | TRANSDUCTION | - |
dc.subject | PATHWAY | - |
dc.subject | INHIBITION | - |
dc.subject | EXPRESSION | - |
dc.title | Nonstructural 5A protein activates beta-catenin signaling cascades: Implication of hepatitis C virus-induced liver pathogenesis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ahn, Byung-Yoon | - |
dc.identifier.doi | 10.1016/j.jhep.2009.06.026 | - |
dc.identifier.scopusid | 2-s2.0-70349735843 | - |
dc.identifier.wosid | 000271760800004 | - |
dc.identifier.bibliographicCitation | JOURNAL OF HEPATOLOGY, v.51, no.5, pp.853 - 864 | - |
dc.relation.isPartOf | JOURNAL OF HEPATOLOGY | - |
dc.citation.title | JOURNAL OF HEPATOLOGY | - |
dc.citation.volume | 51 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 853 | - |
dc.citation.endPage | 864 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.subject.keywordPlus | HUMAN HEPATOCELLULAR CARCINOMAS | - |
dc.subject.keywordPlus | GLYCOGEN-SYNTHASE KINASE-3 | - |
dc.subject.keywordPlus | NS5A PROTEIN | - |
dc.subject.keywordPlus | RNA REPLICATION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | TRANSDUCTION | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | Hepatitis C virus | - |
dc.subject.keywordAuthor | Liver pathogenesis | - |
dc.subject.keywordAuthor | NS5A protein | - |
dc.subject.keywordAuthor | beta-Catenin signaling | - |
dc.subject.keywordAuthor | Tumorigenesis | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.