Heparinized Micropatterned Surfaces for the Spatial Control of Human Mesenchymal Stem Cells
- Authors
- Bae, Jin Woo; Choi, Jong Hoon; Kim, Tae Eun; Park, Ki Dong; Kim, Ji Youn; Park, Yong Doo; Sun, Kyung
- Issue Date
- 11월-2009
- Publisher
- SAGE PUBLICATIONS LTD
- Keywords
- surface-micropatterning cells; heparin; PEGMA photograft; human mesenchymal stem cells; basic fibroblast growth factor; tissue engineering
- Citation
- JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, v.24, no.6, pp.493 - 506
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
- Volume
- 24
- Number
- 6
- Start Page
- 493
- End Page
- 506
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/118988
- DOI
- 10.1177/0883911509349143
- ISSN
- 0883-9115
- Abstract
- In this study, a heparinized micropattern surface was prepared for the spatial control of human mesenchymal stem cells (hMSCs) that can differentiate into the desired tissues. Poly(styrene-co-vinylbenzyl N,N-diethyl dithiocarbamate) (poly(ST-co-VBDC)) was synthesized as a photoreactive polymer; poly(ethylene glycol) methacrylate (PEGMA) was polymerized on the poly(ST-co-VBDC) coated surface by UV irradiation. XPS spectra revealed the residual DC moieties on the PEGMA-grafted surface and the linear chain growth of PEGMA was monitored according to irradiation time. After chemical immobilization of heparin onto this PEGMA surface, surface micropatterning was carried out by additional photopolymerization of PEGMA using a photomask. After incubation for 4 hour, the hMSCs adhered to the heparinized surface, while the hydrophilic PEGMA surface demonstrated no cell adhesion even after basic fibroblast growth factor (bFGF) treatment. Good alignment of hMSCs on the pattern-surface was distinctly observed along micron-sized grooves due to the presence of both heparin and bFGF. This heparinized micropattern surface can be used to study in vitro hMSCs responses with various heparin-binding growth factors in tissue engineering fields as well as cellular array for the spatial control of hMSCs.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.