Crystallization and preliminary X-ray crystallographic analysis of free methionine-(R)-sulfoxide reductase from Staphylococcus aureus
DC Field | Value | Language |
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dc.contributor.author | Bong, Seoung Min | - |
dc.contributor.author | Moon, Jin Ho | - |
dc.contributor.author | Kim, Hwa Young | - |
dc.contributor.author | Kim, Hong Seok | - |
dc.contributor.author | Chi, Young Min | - |
dc.contributor.author | Kim, Augustine Yonghwi | - |
dc.date.accessioned | 2021-09-08T11:59:19Z | - |
dc.date.available | 2021-09-08T11:59:19Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2009-11 | - |
dc.identifier.issn | 2053-230X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/118999 | - |
dc.description.abstract | Free methionine-(R)-sulfoxide reductase (fRMsr) catalyzes the reduction of the free form of methionine-(R)-sulfoxide back to free methionine. The fRMsr protein from Staphylococcus aureus was overexpressed in Escherichia coli, purified and crystallized at 295 K using ammonium sulfate as a precipitant. Diffraction data were collected to 1.7 angstrom resolution from a native crystal using synchrotron radiation. The crystal belonged to the hexagonal space group P6(1)22, with unit-cell parameters a = b = 89.84, c = 88.75 angstrom, alpha = beta = 90, gamma = 120 degrees. Assuming the presence of one molecule in the asymmetric unit, the calculated Matthews coefficient value was 2.21 angstrom(3) Da(-1), with a solvent content of 57.1%. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | INT UNION CRYSTALLOGRAPHY | - |
dc.subject | METHIONINE SULFOXIDE REDUCTASES | - |
dc.subject | PROTEINS | - |
dc.title | Crystallization and preliminary X-ray crystallographic analysis of free methionine-(R)-sulfoxide reductase from Staphylococcus aureus | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chi, Young Min | - |
dc.identifier.doi | 10.1107/S1744309109037105 | - |
dc.identifier.scopusid | 2-s2.0-73449089741 | - |
dc.identifier.wosid | 000271421800011 | - |
dc.identifier.bibliographicCitation | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, v.65, pp.1120 - 1122 | - |
dc.relation.isPartOf | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | - |
dc.citation.title | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | - |
dc.citation.volume | 65 | - |
dc.citation.startPage | 1120 | - |
dc.citation.endPage | 1122 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Crystallography | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Crystallography | - |
dc.subject.keywordPlus | METHIONINE SULFOXIDE REDUCTASES | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordAuthor | Free methionine | - |
dc.subject.keywordAuthor | Free methionine-(R)-sulfoxide reductase | - |
dc.subject.keywordAuthor | Methionine sulfoxide | - |
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