The Pepper Calmodulin Gene CaCaM1 Is Involved in Reactive Oxygen Species and Nitric Oxide Generation Required for Cell Death and the Defense Response
DC Field | Value | Language |
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dc.contributor.author | Choi, Hyong Woo | - |
dc.contributor.author | Lee, Dong Hyuk | - |
dc.contributor.author | Hwang, Byung Kook | - |
dc.date.accessioned | 2021-09-08T12:05:06Z | - |
dc.date.available | 2021-09-08T12:05:06Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2009-11 | - |
dc.identifier.issn | 0894-0282 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/119032 | - |
dc.description.abstract | Calcium signaling has emerged as an important signal transduction pathway of higher plants in response to biotic and abiotic stresses. Ca2+-bound calmodulin (CaM) plays a critical role in decoding and transducing stress signals by activating specific targets. Here, we isolated and functionally characterized the pathogen-responsive CaM gene, Capsicum annuum calmodulin I (CaCaM1), from pepper (C. annuum) plants. The cellular function of CaCaM1 was verified by Agrobacterium spp.-mediated transient expression in pepper and transgenic overexpression in Arabidopsis thaliana. Agrobacterium spp.-mediated transient expression of CaCaM1 activated reactive oxygen species (ROS), nitric oxide (NO) generation, and hypersensitive response (HR)-like cell death in pepper leaves, ultimately leading to local acquired resistance to Xanthomonas campestris pv. vesicatoria. CaCaM1-overexpression (OX) Arabidopsis exhibited enhanced resistance to Pseudomonas syringae and Hyaloperonospora parasitica, which was accompanied by enhanced ROS and NO generation and HR-like cell death. Treatment with the calcium-channel blocker suppressed the oxidative and NO bursts and HR-like cell death that were triggered by CaCaM1 expression in pepper and Arabidopsis, suggesting that calcium influx is required for the activation of CaCaM1-mediated defense responses in plants. Upon treatment with the CaM antagonist, virulent P syringae pv. tomato-induced NO generation was also compromised in CaCaM1-OX leaves. Together, these results suggest that the CaCaM1 gene functions in ROS and NO generation are essential for cell death and defense responses in plants. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER PHYTOPATHOLOGICAL SOC | - |
dc.subject | SIGNALING PATHWAYS | - |
dc.subject | PROTEIN-KINASES | - |
dc.subject | PLANT DEFENSE | - |
dc.subject | CROSS-TALK | - |
dc.subject | HYPERSENSITIVE RESPONSE | - |
dc.subject | CALCIUM-CALMODULIN | - |
dc.subject | STRESS RESPONSES | - |
dc.subject | FUNCTIONAL ROLES | - |
dc.subject | OXIDATIVE BURST | - |
dc.subject | BINDING PROTEIN | - |
dc.title | The Pepper Calmodulin Gene CaCaM1 Is Involved in Reactive Oxygen Species and Nitric Oxide Generation Required for Cell Death and the Defense Response | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Hyong Woo | - |
dc.contributor.affiliatedAuthor | Hwang, Byung Kook | - |
dc.identifier.doi | 10.1094/MPMI-22-11-1389 | - |
dc.identifier.scopusid | 2-s2.0-70349934379 | - |
dc.identifier.wosid | 000271045100007 | - |
dc.identifier.bibliographicCitation | MOLECULAR PLANT-MICROBE INTERACTIONS, v.22, no.11, pp.1389 - 1400 | - |
dc.relation.isPartOf | MOLECULAR PLANT-MICROBE INTERACTIONS | - |
dc.citation.title | MOLECULAR PLANT-MICROBE INTERACTIONS | - |
dc.citation.volume | 22 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1389 | - |
dc.citation.endPage | 1400 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.subject.keywordPlus | SIGNALING PATHWAYS | - |
dc.subject.keywordPlus | PROTEIN-KINASES | - |
dc.subject.keywordPlus | PLANT DEFENSE | - |
dc.subject.keywordPlus | CROSS-TALK | - |
dc.subject.keywordPlus | HYPERSENSITIVE RESPONSE | - |
dc.subject.keywordPlus | CALCIUM-CALMODULIN | - |
dc.subject.keywordPlus | STRESS RESPONSES | - |
dc.subject.keywordPlus | FUNCTIONAL ROLES | - |
dc.subject.keywordPlus | OXIDATIVE BURST | - |
dc.subject.keywordPlus | BINDING PROTEIN | - |
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