Direct Multiplex Reverse Transcription-Nested PCR Detection of Influenza Viruses Without RNA Purification
DC Field | Value | Language |
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dc.contributor.author | Song, Man Ki | - |
dc.contributor.author | Chang, Jun | - |
dc.contributor.author | Hong, Yeongjin | - |
dc.contributor.author | Hong, Sunghoi | - |
dc.contributor.author | Kim, Suhng Wook | - |
dc.date.accessioned | 2021-09-08T12:19:07Z | - |
dc.date.available | 2021-09-08T12:19:07Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2009-11 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/119066 | - |
dc.description.abstract | This paper describes the development a of direct multiplex reverse transcription-nested polymerase chain reaction (PCR) method, devised for simultaneous detection and typing of influenza viruses. This method combines the direct reverse transcription reaction without RNA purification with the enhancement of sensitivity and specificity of nested PCR. The method successfully detected three major human influenza viruses: influenza virus A subtype 1 (H1N1) and subtype 3 (H3N2), and influenza B virus (B). The minimum number of virus particles (pfu/ml) necessary for detection in spiked saliva samples was 200 (H1N1), 140 (H3N2), and 4.5 (B). The method's sensitivity and simplicity will be convenient for use in clinical laboratories for the detection and subtyping of influenza and possibly other RNA viruses. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | MEDIATED ISOTHERMAL AMPLIFICATION | - |
dc.subject | TIME RT-PCR | - |
dc.subject | MORTALITY SURVEILLANCE | - |
dc.subject | RAPID DETECTION | - |
dc.subject | SPECIMENS | - |
dc.subject | PNEUMONIA | - |
dc.subject | DEATHS | - |
dc.subject | SYSTEM | - |
dc.title | Direct Multiplex Reverse Transcription-Nested PCR Detection of Influenza Viruses Without RNA Purification | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hong, Sunghoi | - |
dc.contributor.affiliatedAuthor | Kim, Suhng Wook | - |
dc.identifier.doi | 10.4014/jmb.0905.05012 | - |
dc.identifier.scopusid | 2-s2.0-71549140668 | - |
dc.identifier.wosid | 000272302200027 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.19, no.11, pp.1470 - 1474 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 19 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1470 | - |
dc.citation.endPage | 1474 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001390695 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | MEDIATED ISOTHERMAL AMPLIFICATION | - |
dc.subject.keywordPlus | TIME RT-PCR | - |
dc.subject.keywordPlus | MORTALITY SURVEILLANCE | - |
dc.subject.keywordPlus | RAPID DETECTION | - |
dc.subject.keywordPlus | SPECIMENS | - |
dc.subject.keywordPlus | PNEUMONIA | - |
dc.subject.keywordPlus | DEATHS | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordAuthor | Influenza virus | - |
dc.subject.keywordAuthor | multiplex RT-PCR | - |
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