A simple technique to convert sitting-drop vapor diffusion into hanging-drop vapor diffusion by solidifying the reservoir solution with agarose
- Authors
- Whon, Tae Woong; Lee, Yong-Hwan; An, Dong-Shan; Song, Hyun Kyu; Kim, Song-Gun
- Issue Date
- 10월-2009
- Publisher
- WILEY-BLACKWELL PUBLISHING, INC
- Keywords
- Agarose gel; Crystallization; Glucose isomerase; Lysozyme; Protein crystallography; Solidified reservoirs; Thaumatin; Upside-down sitting-drop vapor diffusion
- Citation
- JOURNAL OF APPLIED CRYSTALLOGRAPHY, v.42, pp.975 - 976
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF APPLIED CRYSTALLOGRAPHY
- Volume
- 42
- Start Page
- 975
- End Page
- 976
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/119290
- DOI
- 10.1107/S0021889809028805
- ISSN
- 0021-8898
- Abstract
- A simple protocol to convert sitting-drop vapor-diffusion plating into a hanging-drop vapor-diffusion experiment in protein crystallization is reported. After making a sitting-drop plate, agarose solution was added to solidify the reservoir solution, and the plates were incubated upside down. Crystallization experiments with hen egg white lysozyme, thaumatin and glucose isomerase showed that the 'upside-down sitting-drop' method could produce single crystals with all the benefits of the hanging-drop crystallization method.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Life Sciences > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.