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Electrochemical Detection of Single Nucleotide Polymorphism using Gold Electrode Arrays Modified with Thiol Linkers Synthesized by Solid-Phase Strategy

Authors
Lee, Sang-MyungKang, Eun-KyungShin, Dong-SikCho, Hong-JunKim, Jung-SeokChoi, Yon-SikPak, James JunghoPak, Youngmi KimLee, Yoon-Sik
Issue Date
20-9월-2009
Publisher
KOREAN BIOCHIP SOCIETY-KBCS
Keywords
Electrochemical DNA sensor; Gold electrode arrays; Mitochondrial DNA; Single nucleotide polymorphism; Solid-phase synthesis
Citation
BIOCHIP JOURNAL, v.3, no.3, pp.266 - 274
Indexed
SCIE
SCOPUS
KCI
OTHER
Journal Title
BIOCHIP JOURNAL
Volume
3
Number
3
Start Page
266
End Page
274
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/119307
ISSN
1976-0280
Abstract
We developed an electrochemical DNA sensor based on gold electrode arrays modified with a thiol linker synthesized by novel solid-phase strategy, and could effectively detect the aging-dependant T414G mutation of mitochondrial DNA. To electrochemically detect the single nucleotide polymorphism (SNP), the gold electrode arrays were patterned on glass wafers, and the thiol linker facilely synthesized on the solid support was assembled on the gold electrode surface. On the SAM electrode surface, three kinds of DNA probes, T414G-T probe, T414G-G probe and control R probe, were covalently immobilized. Using the cyclic voltametry (CV), we could not only discriminate between full-matched DNA and single-mismatched DNA successfully, but also confirm the effectiveness of the synthesized thiol linkers for reducing the nonspecific DNA binding.
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Pak, James Jung ho
공과대학 (전기전자공학부)
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