Absolute CD4(+) Cell Count Using a Plastic Microchip and a Microscopic Cell Counter
DC Field | Value | Language |
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dc.contributor.author | Bae, Sook Young | - |
dc.contributor.author | Park, Hae Chul | - |
dc.contributor.author | Oh, Jun Seo | - |
dc.contributor.author | Yoon, Soo-Young | - |
dc.contributor.author | Park, Dae Won | - |
dc.contributor.author | Choi, In Keun | - |
dc.contributor.author | Kim, Hae Joong | - |
dc.contributor.author | Oh, Jong Hyun | - |
dc.contributor.author | Hur, Dae Sung | - |
dc.contributor.author | Chung, Chanil | - |
dc.contributor.author | Chang, Jun Keun | - |
dc.contributor.author | Robinson, J. Paul | - |
dc.contributor.author | Lim, Chae Seung | - |
dc.date.accessioned | 2021-09-08T13:45:48Z | - |
dc.date.available | 2021-09-08T13:45:48Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2009-09 | - |
dc.identifier.issn | 1552-4949 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/119356 | - |
dc.description.abstract | We have designed and evaluated the performance of a simple, rapid, and affordable method for counting CD4(+) T-cells with the use of plastic microchips. This new system is an adaptation of a "no-lyse, no-wash," volumetric single platform assay, and absolute CD4(+) counts are determined with the use of a microscopic scanning cell counter. To assess the CD4(+) count test precision and linearity of the system, measured CD4(+) counts were compared with two other reference assays (single and dual platform flow cytometry) with the use of 123 clinical samples including samples obtained from 35 HIV-infected patients, and artificially diluted samples. A correlation between the results from the use of the new method and from the use of the two other reference assays was r = 0.98 for the clinical samples. A dilution test of the new method demonstrated a linearity of r >= 0.99, with coefficients of variation <= 7.6% for all concentration levels. Our findings suggest that the new CD4(+) counting device can be potentially be applied for other diagnostic procedures that measure quantities of characteristic antigens or other materials on cells. (C) 2009 Clinical Cytometry Society | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | PLATFORM IMAGE CYTOMETER | - |
dc.subject | VOLUMETRIC CAPILLARY CYTOMETRY | - |
dc.subject | HUMAN-IMMUNODEFICIENCY-VIRUS | - |
dc.subject | RESOURCE-POOR SETTINGS | - |
dc.subject | T-LYMPHOCYTES | - |
dc.subject | HIV | - |
dc.subject | ENUMERATION | - |
dc.subject | BLOOD | - |
dc.subject | QUANTITATION | - |
dc.subject | NUMBERS | - |
dc.title | Absolute CD4(+) Cell Count Using a Plastic Microchip and a Microscopic Cell Counter | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Hae Chul | - |
dc.contributor.affiliatedAuthor | Oh, Jun Seo | - |
dc.contributor.affiliatedAuthor | Yoon, Soo-Young | - |
dc.contributor.affiliatedAuthor | Park, Dae Won | - |
dc.contributor.affiliatedAuthor | Choi, In Keun | - |
dc.contributor.affiliatedAuthor | Kim, Hae Joong | - |
dc.contributor.affiliatedAuthor | Lim, Chae Seung | - |
dc.identifier.doi | 10.1002/cyto.b.20470 | - |
dc.identifier.scopusid | 2-s2.0-74849096381 | - |
dc.identifier.wosid | 000269426400006 | - |
dc.identifier.bibliographicCitation | CYTOMETRY PART B-CLINICAL CYTOMETRY, v.76B, no.5, pp.345 - 353 | - |
dc.relation.isPartOf | CYTOMETRY PART B-CLINICAL CYTOMETRY | - |
dc.citation.title | CYTOMETRY PART B-CLINICAL CYTOMETRY | - |
dc.citation.volume | 76B | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 345 | - |
dc.citation.endPage | 353 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Medical Laboratory Technology | - |
dc.relation.journalResearchArea | Pathology | - |
dc.relation.journalWebOfScienceCategory | Medical Laboratory Technology | - |
dc.relation.journalWebOfScienceCategory | Pathology | - |
dc.subject.keywordPlus | PLATFORM IMAGE CYTOMETER | - |
dc.subject.keywordPlus | VOLUMETRIC CAPILLARY CYTOMETRY | - |
dc.subject.keywordPlus | HUMAN-IMMUNODEFICIENCY-VIRUS | - |
dc.subject.keywordPlus | RESOURCE-POOR SETTINGS | - |
dc.subject.keywordPlus | T-LYMPHOCYTES | - |
dc.subject.keywordPlus | HIV | - |
dc.subject.keywordPlus | ENUMERATION | - |
dc.subject.keywordPlus | BLOOD | - |
dc.subject.keywordPlus | QUANTITATION | - |
dc.subject.keywordPlus | NUMBERS | - |
dc.subject.keywordAuthor | antigens | - |
dc.subject.keywordAuthor | CD4 | - |
dc.subject.keywordAuthor | CD4 lymphocyte count | - |
dc.subject.keywordAuthor | flow cytometry | - |
dc.subject.keywordAuthor | microchip | - |
dc.subject.keywordAuthor | absolute CD4 count | - |
dc.subject.keywordAuthor | microscopic cell counter | - |
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