DNA profiling by array comparative genomic hybridization (CGH) of peripheral blood mononuclear cells (PBMC) and tumor tissue cell in non-small cell lung cancer (NSCLC)
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Baik, Seung-Ho | - |
dc.contributor.author | Jee, Bo-Keun | - |
dc.contributor.author | Choi, Jin-Soo | - |
dc.contributor.author | Yoon, Hyoung-Kyu | - |
dc.contributor.author | Lee, Kweon-Haeng | - |
dc.contributor.author | Kim, Yeul-Hong | - |
dc.contributor.author | Lim, Young | - |
dc.date.accessioned | 2021-09-08T14:16:55Z | - |
dc.date.available | 2021-09-08T14:16:55Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2009-09 | - |
dc.identifier.issn | 0301-4851 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/119465 | - |
dc.description.abstract | Lung tumor cell DNA copy number alteration (CNA) was expected to display specific patterns such as a large-scale amplification or deletion of chromosomal arms, as previously published data have reported. Peripheral blood mononuclear cell (PBMC) CNA however, was expected to show normal variations in cancer patients as well as healthy individuals, and has thus been used as normal control DNA samples in various published studies. We performed array CGH to measure and compare genetic changes in terms of the CNA of PBMC samples as well as DNA isolated from tumor tissue samples, obtained from 24 non-small cell lung cancer patients. Contradictory to expectations, our studies showed that the PBMC CNA also showed chromosomal variant regions. The list included well-known tumor-associated NTRK1, FGF8, TP53, and TGF beta 1 genes and potentially novel oncogenes such as THPO (3q27.1), JMJD1B, and EGR1 (5q31.2), which was investigated in this study. The results of this study highlighted the connection between PBMC and tumor cell genomic DNA in lung cancer patients. However, the application of these studies to cancer prognosis may pose a challenge due to the large amount of information contained in genetic predisposition and family history that has to be processed for useful downstream clinical applications. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | EPIDERMAL GROWTH-FACTOR | - |
dc.subject | COPY NUMBER ALTERATION | - |
dc.subject | REAL-TIME PCR | - |
dc.subject | FINGER TRANSCRIPTION FACTOR | - |
dc.subject | HIGH-RESOLUTION ANALYSIS | - |
dc.subject | BREAST-CANCER | - |
dc.subject | UROTHELIAL CANCERS | - |
dc.subject | COLORECTAL-CANCER | - |
dc.subject | BRAIN-TUMORS | - |
dc.subject | FACTOR EGR-1 | - |
dc.title | DNA profiling by array comparative genomic hybridization (CGH) of peripheral blood mononuclear cells (PBMC) and tumor tissue cell in non-small cell lung cancer (NSCLC) | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Yeul-Hong | - |
dc.identifier.doi | 10.1007/s11033-008-9380-7 | - |
dc.identifier.scopusid | 2-s2.0-68149125221 | - |
dc.identifier.wosid | 000268496800016 | - |
dc.identifier.bibliographicCitation | MOLECULAR BIOLOGY REPORTS, v.36, no.7, pp.1767 - 1778 | - |
dc.relation.isPartOf | MOLECULAR BIOLOGY REPORTS | - |
dc.citation.title | MOLECULAR BIOLOGY REPORTS | - |
dc.citation.volume | 36 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1767 | - |
dc.citation.endPage | 1778 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | EPIDERMAL GROWTH-FACTOR | - |
dc.subject.keywordPlus | COPY NUMBER ALTERATION | - |
dc.subject.keywordPlus | REAL-TIME PCR | - |
dc.subject.keywordPlus | FINGER TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | HIGH-RESOLUTION ANALYSIS | - |
dc.subject.keywordPlus | BREAST-CANCER | - |
dc.subject.keywordPlus | UROTHELIAL CANCERS | - |
dc.subject.keywordPlus | COLORECTAL-CANCER | - |
dc.subject.keywordPlus | BRAIN-TUMORS | - |
dc.subject.keywordPlus | FACTOR EGR-1 | - |
dc.subject.keywordAuthor | Copy number alteration | - |
dc.subject.keywordAuthor | Peripheral blood mononuclear cell | - |
dc.subject.keywordAuthor | Non-small cell lung cancer | - |
dc.subject.keywordAuthor | Array CGH | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.