Reinvestigation of the Molecular Influence of Hypoxanthine on the DNA Cleavage Efficiency of Restriction Endonucleases BglII, EcoRI and BamHI
DC Field | Value | Language |
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dc.contributor.author | Doi, Akihiro | - |
dc.contributor.author | Pack, Seung Pil | - |
dc.contributor.author | Kodaki, Tsutomu | - |
dc.contributor.author | Makino, Keisuke | - |
dc.date.accessioned | 2021-09-08T15:09:17Z | - |
dc.date.available | 2021-09-08T15:09:17Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2009-08 | - |
dc.identifier.issn | 0021-924X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/119577 | - |
dc.description.abstract | Hypoxanthine (Hyp), a deaminated base of adenine (Ade), can be employed as a good probe molecule to reveal the significance of the minor groove of guanine (Gua) in biomolecular interactions because Hyp possesses a similar structure to Gua lacking its 2-amino group. In this study, we examined cleavage efficiencies of restriction endonuclease enzymes on DNA substrates with Hyp in their recognition sequences. As a substrate for BglII, EcoRI and BamHI, 24-mer DNA oligomer with Hyp (in place of Gua) was prepared together with its complementary sequences with cytosine (Cyt) or thymine (Thy) as the counter base. At 37 degrees C incubation for 1h, BglII and EcoRI showed higher DNA cleavage reactivity on Hyp-containing DNA substrates than on normal ones, whereas BamHI showed lower values on Hyp-containing substrates. Such high cleavage performance of BglII and EcoRI on Hyp-containing DNA substrates is in contrast to the results obtained 20 years ago, in which short DNA substrates (8- or 10-mer) and low reaction temperatures (15-20 degrees C) were employed. These new results suggest that the lack of the exocyclic 2-amino group of Gua could contribute to enhanced recognition access of BglII and EcoRI to DNA substrates. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.subject | DEOXYINOSINE | - |
dc.subject | PROBE | - |
dc.subject | BASES | - |
dc.subject | ACID | - |
dc.title | Reinvestigation of the Molecular Influence of Hypoxanthine on the DNA Cleavage Efficiency of Restriction Endonucleases BglII, EcoRI and BamHI | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Pack, Seung Pil | - |
dc.identifier.doi | 10.1093/jb/mvp060 | - |
dc.identifier.scopusid | 2-s2.0-68649083516 | - |
dc.identifier.wosid | 000268812700007 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOCHEMISTRY, v.146, no.2, pp.201 - 208 | - |
dc.relation.isPartOf | JOURNAL OF BIOCHEMISTRY | - |
dc.citation.title | JOURNAL OF BIOCHEMISTRY | - |
dc.citation.volume | 146 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 201 | - |
dc.citation.endPage | 208 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | DEOXYINOSINE | - |
dc.subject.keywordPlus | PROBE | - |
dc.subject.keywordPlus | BASES | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordAuthor | BglII | - |
dc.subject.keywordAuthor | EcoRI | - |
dc.subject.keywordAuthor | hypoxanthine | - |
dc.subject.keywordAuthor | minor groove | - |
dc.subject.keywordAuthor | restriction endonuclease | - |
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