Nanomechanical In Situ Monitoring of Proteolysis of Peptide by Cathepsin B
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwon, Taeyun | - |
dc.contributor.author | Park, Jinsung | - |
dc.contributor.author | Yang, Jaemoon | - |
dc.contributor.author | Yoon, Dae Sung | - |
dc.contributor.author | Na, Sungsoo | - |
dc.contributor.author | Kim, Chang-Wan | - |
dc.contributor.author | Suh, Jin-Suck | - |
dc.contributor.author | Huh, Yong-Min | - |
dc.contributor.author | Haam, Seungjoo | - |
dc.contributor.author | Eom, Kilho | - |
dc.date.accessioned | 2021-09-08T15:28:31Z | - |
dc.date.available | 2021-09-08T15:28:31Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2009-07-16 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/119663 | - |
dc.description.abstract | Characterization and control of proteolysis of peptides by specific cellular protease is a priori requisite for effective drug discovery. Here, we report the nanomechanical, in situ monitoring of proteolysis of peptide chain attributed to protease (Cathepsin B) by using a resonant nanomechanical microcantilever immersed in a liquid. Specifically, the detection is based on measurement of resonant frequency shift arising from proteolysis of peptides (leading to decrease of cantilever's overall mass, and consequently, increases in the resonance). It is shown that resonant microcantilever enables the quantification of proteolysis efficacy with respect to protease concentration. Remarkably, the nanomechanical, in situ monitoring of proteolysis allows us to gain insight into the kinetics of proteolysis of peptides, which is well depicted by Langmuir kinetic model. This implies that nanomechanical biosensor enables the characterization of specific cellular protease such as its kinetics. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.title | Nanomechanical In Situ Monitoring of Proteolysis of Peptide by Cathepsin B | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Na, Sungsoo | - |
dc.contributor.affiliatedAuthor | Eom, Kilho | - |
dc.identifier.doi | 10.1371/journal.pone.0006248 | - |
dc.identifier.scopusid | 2-s2.0-67650930365 | - |
dc.identifier.wosid | 000268036000007 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.4, no.7 | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 4 | - |
dc.citation.number | 7 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordAuthor | Nanomechanical Detection | - |
dc.subject.keywordAuthor | Proteolysis | - |
dc.subject.keywordAuthor | Enzyme | - |
dc.subject.keywordAuthor | Kinetics | - |
dc.subject.keywordAuthor | Cantilever | - |
dc.subject.keywordAuthor | Bioassay | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.