Development of a Saccharomyces cerevisiae strain for the production of 1,2-propanediol by gene manipulation
DC Field | Value | Language |
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dc.contributor.author | Jeon, Eunyoung | - |
dc.contributor.author | Lee, Soojin | - |
dc.contributor.author | Kim, Donghyun | - |
dc.contributor.author | Yoon, Hyunsik | - |
dc.contributor.author | Oh, Minkyu | - |
dc.contributor.author | Park, Chulhwan | - |
dc.contributor.author | Lee, Jinwon | - |
dc.date.accessioned | 2021-09-08T15:31:10Z | - |
dc.date.available | 2021-09-08T15:31:10Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2009-07-08 | - |
dc.identifier.issn | 0141-0229 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/119677 | - |
dc.description.abstract | The main goal of this research was to achieve a more efficient production of 1,2-propanediol (1,2-PD) using mutated Saccharomyces cerevisiae. 1,2-PD cannot be produced by wild type S. cerevisiae. To develop a S. cerevisiae mutant that could produce 1,2-PD, the mgs gene of E. coli-K12 MG1655 and the dhaD gene of Citrobacter freundii were inserted into yeast expression vectors such as pESC-URA and pESC-TRP and transformed into the wild type of S. cerevisiae. As a result, the batch fermentation of S. cerevisiae YPH500, harboring an mgs gene inserted pJES27 vector, resulted in a yield of 0.17 g/L. On the other hand, the methylglyoxal synthase of the recombinant S. cerevisiae YPH500, harboring a dhaD gene inserted pJES29 vector, was inactive and produced no detectable amount of 1,2-PD. Therefore, in order to achieve a maximum yield of 1,2-PD, we selected the pESC-TRP vector that is able to co-express the dhaD gene with the pJES27 vector. By inserting the dhaD gene into the pESC-TRP vector, the pJES30 vector was constructed. The maximal yield of 1,2-PD achieved in a 1% galactose batch fermentation by pJES27 and pJES30 harboring S. cerevisiae was 0.45 g/L. (C) 2009 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.subject | YEAST | - |
dc.title | Development of a Saccharomyces cerevisiae strain for the production of 1,2-propanediol by gene manipulation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Minkyu | - |
dc.identifier.doi | 10.1016/j.enzmictec.2009.03.009 | - |
dc.identifier.scopusid | 2-s2.0-67349238242 | - |
dc.identifier.wosid | 000267415100007 | - |
dc.identifier.bibliographicCitation | ENZYME AND MICROBIAL TECHNOLOGY, v.45, no.1, pp.42 - 47 | - |
dc.relation.isPartOf | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.citation.title | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.citation.volume | 45 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 42 | - |
dc.citation.endPage | 47 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | YEAST | - |
dc.subject.keywordAuthor | Saccharomyces cerevisiae | - |
dc.subject.keywordAuthor | 1,2-Propanediol | - |
dc.subject.keywordAuthor | Mgs gene | - |
dc.subject.keywordAuthor | DhaD gene | - |
dc.subject.keywordAuthor | Fermentation | - |
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