Proteolysis and synthetic strategy of human G-CSF in Escherichia coli BL21 (DE3)
DC Field | Value | Language |
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dc.contributor.author | Song, Jong-Am | - |
dc.contributor.author | Han, Kyung-Yeon | - |
dc.contributor.author | Ahn, Keum-Young | - |
dc.contributor.author | Park, Jin-Seung | - |
dc.contributor.author | Seo, Hyuk-Seong | - |
dc.contributor.author | Lee, Jeewon | - |
dc.date.accessioned | 2021-09-08T15:31:21Z | - |
dc.date.available | 2021-09-08T15:31:21Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2009-07-08 | - |
dc.identifier.issn | 0141-0229 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/119678 | - |
dc.description.abstract | We report for the first time that the C-terminal region of hG-CSF suffers from proteolytic degradation when human granulocyte colony-stimulating factor (hG-CSF) is directly expressed in Escherichia coli BL21 (DE3). It is believed that the rapid proteolysis occurs at the C-terminus of hG-CSF that is very easily exposed to E. coli protease(s) during a short period following protein synthesis and prior to completion of the formation of the inclusion body. The recombinant hG-CSF that is expressed with an N-terminal fusion partner is effectively protected from the proteolysis. It seems that since the N-terminus of hG-CSF is located very close to the C-terminus, the presence of the N-terminal fusion partner masks the C-terminal region of hG-CSF and protects it from proteolytic degradation by E. coli protease(s). Furthermore, the solubility of hG-CSF markedly increased in E coli cytoplasm when a stress-responsive and aggregation-resistant protein, i.e. aspartate carbamoyl-transferase catalytic chain (PyrB) Was used as a novel N-terminal fusion partner proteins. (C) 2009 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.subject | COLONY-STIMULATING FACTOR | - |
dc.subject | GROWTH-FACTORS | - |
dc.subject | PROTEIN | - |
dc.subject | EXPRESSION | - |
dc.subject | FUSION | - |
dc.subject | PURIFICATION | - |
dc.subject | CHAIN | - |
dc.title | Proteolysis and synthetic strategy of human G-CSF in Escherichia coli BL21 (DE3) | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Jeewon | - |
dc.identifier.doi | 10.1016/j.enzmictec.2009.02.010 | - |
dc.identifier.scopusid | 2-s2.0-67349157187 | - |
dc.identifier.wosid | 000267415100002 | - |
dc.identifier.bibliographicCitation | ENZYME AND MICROBIAL TECHNOLOGY, v.45, no.1, pp.7 - 14 | - |
dc.relation.isPartOf | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.citation.title | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.citation.volume | 45 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 7 | - |
dc.citation.endPage | 14 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | COLONY-STIMULATING FACTOR | - |
dc.subject.keywordPlus | GROWTH-FACTORS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | FUSION | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | CHAIN | - |
dc.subject.keywordAuthor | Human granulocyte colony-stimulating factor (hG-CSF) | - |
dc.subject.keywordAuthor | E. coli BL21(DE3) | - |
dc.subject.keywordAuthor | N-terminal fusion partner | - |
dc.subject.keywordAuthor | C-terminal proteolysis | - |
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