Caffeic acid phenethyl ester accumulates beta-catenin through GSK-3 beta and participates in proliferation through mTOR in C2C12 cells
- Authors
- Lee, Eun Soo; Lee, Jung-Ok; Lee, Soo Kyung; Kim, Ji Hae; Jung, Jin Hee; Keum, Bora; Park, Sun-Hwa; Kim, Hyeon Soo
- Issue Date
- 22-5월-2009
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Akt; beta-catenin; CAPE; GSK-3 beta; mTOR
- Citation
- LIFE SCIENCES, v.84, no.21-22, pp.755 - 759
- Indexed
- SCIE
SCOPUS
- Journal Title
- LIFE SCIENCES
- Volume
- 84
- Number
- 21-22
- Start Page
- 755
- End Page
- 759
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/120031
- DOI
- 10.1016/j.lfs.2009.03.004
- ISSN
- 0024-3205
- Abstract
- Aim: The aim of this study is to characterize the roles of caffeic acid phenethyl ester (CAPE) in the skeletal muscle cells. Main methods: We performed immunoblotting assay using various phosphorylation specific antibodies. Key findings: We found that CAPE induces rapid and transient phosphorylation of glycogen synthase kinase (GSK)-3 beta in a phosphoinositide 3-kinase (PI3K)-dependent manner. CAPE also decreases phosphorylation of beta-catenin, ultimately leading to beta-catenin accumulation. In addition, we demonstrated that CAPE activated the mammalian target of rapamycin (mTOR)-p70 S6 ribosomal kinase (S6K) and also stimulated extracellular signal-regulated kinase (ERK). The inhibition of mTOR blocked CAPE-induced ERK phosphorylation. Significance: Our results suggest that CAPE may act through beta-catenin accumulation via stimulation of GSK-3 beta and may also participate in cellular proliferation through the mTOR-ERK pathway. (C) 2009 Elsevier Inc. All rights reserved.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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