Expression of glutaredoxin-1 in nasal polyps and airway epithelial cells
- Authors
- Woo, Hyun-Jae; Bae, Chang Hoon; Song, Si-Youn; Kim, Yong-Woon; Lee, Heung-Man; Kim, Yong-Dae
- Issue Date
- 5월-2009
- Publisher
- SAGE PUBLICATIONS INC
- Keywords
- Airway epithelial cells; glucocorticoids; glutaredoxin-1; interleukin-1 beta; nasal polyps; oxidative stress; reactive oxygen species
- Citation
- AMERICAN JOURNAL OF RHINOLOGY & ALLERGY, v.23, no.3, pp.288 - 293
- Indexed
- SCIE
SCOPUS
- Journal Title
- AMERICAN JOURNAL OF RHINOLOGY & ALLERGY
- Volume
- 23
- Number
- 3
- Start Page
- 288
- End Page
- 293
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/120077
- DOI
- 10.2500/ajra.2009.23.3318
- ISSN
- 1945-8924
- Abstract
- Background: Glutaredoxins (GRX)-1 is glutathione-dependent oxidoreductase. However, the role of these enzymes remains unknown in airway inflammatory diseases. Therefore, we aimed to establish the expression pattern of GRX-1 in the nasal polyps (NPs) and to assess the regulatory mechanisms associated with GRX-1 expression in interleukin (IL)-1beta-treated airway epithelial cells. Methods: The expression of GRX-1 in NPs and normal nasal mucosa were analyzed by reverse-transcription polymerase chain reaction and immunohistochemical staining. IL-1beta-induced reactive oxygen species (ROS) formation and GRX-1 expression in the airway epithelial cells was determined by flow cytometry and immunoassay. Results: The expression level of GRX-1 in NPs was significantly higher than in the normal nasal mucosa (p < 0.05). GRX-1 was highly expressed in the surface epithelial cells and the submucosal glandular cells in the NPs. IL-1beta increased the intracellular ROS formation and GRX-1 expression in airway epithelial cells, The inhibition of IL-1beta-induced ROS production by N-acetyl-cystein, an ROS scavenger, reduced GRX-1 expression. Diphenyleneiodonium and apocynin, NADPH oxidase inhibitors, did not abolish IL-1beta-induced ROS formation and GRX-1 expression, whereas budesonide attenuated it. Conclusion: High GRX-1 expression in NPs might be a primary defense against chronic inflammatory oxidative stress in nasal mucosa. IL-1beta-induced up-regulation of GRX-1 in airway epithelial cells is probably mediated by ROS. Glucocorticoids can regulate IL-1beta-induced ROS formation and GRX-1 expression. (Am J Rhinol Allergy 23, 288-293, 2009; doi: 10.2500/ajra.2009.23.3318)
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