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Proteome analysis of adipocyte lipid rafts reveals that gC1qR plays essential roles in adipogenesis and insulin signal transduction

Authors
Kim, Ki-BumKim, Bong-WooChoo, Hyo-JungKwon, Young-ChanAhn, Byung-YoonChoi, Jong-SoonLee, Jae-SeonKo, Young-Gyu
Issue Date
5월-2009
Publisher
WILEY
Keywords
Insulin resistance; Lipid raft; Signal transduction; Two-dimensional difference gel electrophoresis
Citation
PROTEOMICS, v.9, no.9, pp.2373 - 2382
Indexed
SCIE
SCOPUS
Journal Title
PROTEOMICS
Volume
9
Number
9
Start Page
2373
End Page
2382
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/120157
DOI
10.1002/pmic.200800811
ISSN
1615-9853
Abstract
Since insulin receptors and their downstream signaling molecules are organized in lipid rafts, proteomic analysis of adipocyte lipid rafts may provide new insights into the function of lipid rafts in adipogenesis and insulin signaling. To search for proteins involved in adipocyte differentiation and insulin signaling, we analyzed detergent-resistant lipid raft proteins from 3T3-L1 preadipocytes and adipocytes by 2-DE. Eleven raft proteins were identified from adipocytes. One of the adipocyte-specific proteins was globular C1q receptor (gC1qR), an acidic 32 kDa protein known as the receptor for the globular domain of complement C1q. The targeting of gC1qR into lipid rafts was significantly increased during adipogenesis, as determined by immunoblotting and immunofluorescence. Since the silencing of gC1qR by small RNA interference abolished adipogenesis and blocked insulin-induced activation of insulin receptor, insulin receptor substrate-1 (IRS-1), Akt, and Erk1/2, we can conclude that gC1qR is an essential molecule involved in adipogenesis and insulin signaling.
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