Disruption of Glycosylphosphatidylinositol-Anchored Lipid Transfer Protein Gene Altered Cuticular Lipid Composition, Increased Plastoglobules, and Enhanced Susceptibility to Infection by the Fungal Pathogen Alternaria brassicicola
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Saet Buyl | - |
dc.contributor.author | Go, Young Sam | - |
dc.contributor.author | Bae, Hyun-Jong | - |
dc.contributor.author | Park, Jong Ho | - |
dc.contributor.author | Cho, Sung Ho | - |
dc.contributor.author | Cho, Hong Joo | - |
dc.contributor.author | Lee, Dong Sook | - |
dc.contributor.author | Park, Ohkmae K. | - |
dc.contributor.author | Hwang, Inhwan | - |
dc.contributor.author | Suh, Mi Chung | - |
dc.date.accessioned | 2021-09-08T17:42:20Z | - |
dc.date.available | 2021-09-08T17:42:20Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2009-05 | - |
dc.identifier.issn | 0032-0889 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/120171 | - |
dc.description.abstract | All aerial parts of vascular plants are covered with cuticular waxes, which are synthesized by extensive export of intracellular lipids from epidermal cells to the surface. Although it has been suggested that plant lipid transfer proteins (LTPs) are involved in cuticular lipid transport, the in planta evidence is still not clear. In this study, a glycosylphosphatidylinositol-anchored LTP (LTPG1) showing higher expression in epidermal peels of stems than in stems was identified from an Arabidopsis (Arabidopsis thaliana) genome-wide microarray analysis. The expression of LTPG1 was observed in various tissues, including the epidermis, stem cortex, vascular bundles, mesophyll cells, root tips, pollen, and early-developing seeds. LTPG1 was found to be localized in the plasma membrane. Disruption of the LTPG1 gene caused alterations of cuticular lipid composition, but no significant changes on total wax and cutin monomer loads were seen. The largest reduction (10 mass %) in the ltpg1 mutant was observed in the C29 alkane, which is the major component of cuticular waxes in the stems and siliques. The reduced content was overcome by increases of the C29 secondary alcohols and C29 ketone wax loads. The ultrastructure analysis of ltpg1 showed a more diffuse cuticular layer structure, protrusions of the cytoplasm into the vacuole in the epidermis, and an increase of plastoglobules in the stem cortex and leaf mesophyll cells. Furthermore, the ltpg1 mutant was more susceptible to infection by the fungus Alternaria brassicicola than the wild type. Taken together, these results indicated that LTPG1 contributed either directly or indirectly to cuticular lipid accumulation. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC PLANT BIOLOGISTS | - |
dc.subject | FATTY-ACID ELONGASE | - |
dc.subject | WAX BIOSYNTHESIS | - |
dc.subject | ABC TRANSPORTER | - |
dc.subject | ARABIDOPSIS-THALIANA | - |
dc.subject | EXPRESSION | - |
dc.subject | IDENTIFICATION | - |
dc.subject | CUTICLE | - |
dc.subject | ENCODES | - |
dc.subject | FUSION | - |
dc.subject | POLLEN | - |
dc.title | Disruption of Glycosylphosphatidylinositol-Anchored Lipid Transfer Protein Gene Altered Cuticular Lipid Composition, Increased Plastoglobules, and Enhanced Susceptibility to Infection by the Fungal Pathogen Alternaria brassicicola | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Ohkmae K. | - |
dc.identifier.doi | 10.1104/pp.109.137745 | - |
dc.identifier.scopusid | 2-s2.0-66149095028 | - |
dc.identifier.wosid | 000265661700005 | - |
dc.identifier.bibliographicCitation | PLANT PHYSIOLOGY, v.150, no.1, pp.42 - 54 | - |
dc.relation.isPartOf | PLANT PHYSIOLOGY | - |
dc.citation.title | PLANT PHYSIOLOGY | - |
dc.citation.volume | 150 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 42 | - |
dc.citation.endPage | 54 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.subject.keywordPlus | FATTY-ACID ELONGASE | - |
dc.subject.keywordPlus | WAX BIOSYNTHESIS | - |
dc.subject.keywordPlus | ABC TRANSPORTER | - |
dc.subject.keywordPlus | ARABIDOPSIS-THALIANA | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | CUTICLE | - |
dc.subject.keywordPlus | ENCODES | - |
dc.subject.keywordPlus | FUSION | - |
dc.subject.keywordPlus | POLLEN | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.