Combinations of Growth Factors Enhance the Potency of Islets In Vitro
- Authors
- Lim, Jong Yeon; Min, Byoung Hoon; Kim, Byoung Geun; Shin, Jun-Seop; Park, Chang Sook; Yoon, Tai Wook; Han, Sung Sik
- Issue Date
- 5월-2009
- Publisher
- LIPPINCOTT WILLIAMS & WILKINS
- Keywords
- islet; growth factor; proliferation; differentiation; transplantation
- Citation
- PANCREAS, v.38, no.4, pp.447 - 453
- Indexed
- SCIE
SCOPUS
- Journal Title
- PANCREAS
- Volume
- 38
- Number
- 4
- Start Page
- 447
- End Page
- 453
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/120176
- DOI
- 10.1097/MPA.0b013e318197a62e
- ISSN
- 0885-3177
- Abstract
- Objectives: Recent studies have demonstrated the impressive expansion of A cells in vitro. But unfortunately, expanded A cells do not function in the same way as fully differentiated A cells. Therefore, we developed a condition that would allow islet cells to proliferate while maintaining their endocrine function. Methods: We tested the different use of growth factors in a different culture period. And we tested the possibility of adult islets, which expanded during a short period, as a clinical source of islet cells by comparing the efficiency of transplantation of cultured islets with that of fresh islets. Results: The islets showed a time-dependent increase in proliferative activity, reaching 32.2% on day 5. After 5 days of culture, the efficiency of transplantation of cultured islets was increased (2-fold) in comparison to that of noncultured islets. Moreover, islet transplantation immediately induced normoglycemia at a level equal to native islets. Conclusions: These findings suggest that adult A cells have the potential to proliferate while maintaining their endocrine function, which can be improved through careful regulation of proliferation and differentiation.
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Collections - College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles
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