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Highly Sensitive Immunoassay of Lung Cancer Marker Carcinoembryonic Antigen Using Surface-Enhanced Raman Scattering of Hallow Gold Nanospheres

Authors
Chon, HyangahLee, SangyeopSon, Sang WookOh, Chil HwanChoo, Jaebum
Issue Date
15-4월-2009
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, v.81, no.8, pp.3029 - 3034
Indexed
SCIE
SCOPUS
Journal Title
ANALYTICAL CHEMISTRY
Volume
81
Number
8
Start Page
3029
End Page
3034
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/120238
DOI
10.1021/ac802722c
ISSN
0003-2700
Abstract
A quick and reproducible surface-enhanced Raman scattering (SERS)-based immunoassay technique, using hollow gold nanospheres (HGNs) and magnetic beads, has been developed. Here, HGNs show strong enhancement effects from individual particles because hot spots can be localized on the pinholes in the hollow particle structure. Thus, HGNs can be used for highly reproducible immunoanalysis of cancer markers. Magnetic beads were used as supporting substrates for the formation of the immunocomplex. This SERS-based immunoassay technique overcomes the problem of slow immunoreaction caused by the diffusion-limited kinetics on a solid substrate because all of the reactions occur in solution. For the validation of our SERS immunoassay, a well-known lung cancer marker, carcinoembryonic antigen (CEA), was used as a target marker. According to our experimental results, the limit of detection (LOD) was determined to be 1-10 pg/mL, this value being about 100-1000 times more sensitive than the LOD of enzyme-linked immunosorbent assay. Furthermore, the assay time took less than 1 h, including washing and optical detection steps.
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