Stabilization of the immobilized linkers and DNA probes for DNA microarray fabrication by end-capping of the remaining unreacted silanol on the glass
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kamisetty, Nagendra Kumar | - |
dc.contributor.author | Pack, Seung Pil | - |
dc.contributor.author | Nonogawa, Mitsuru | - |
dc.contributor.author | Yamada, Kazunari | - |
dc.contributor.author | Yoshida, Yasuko | - |
dc.contributor.author | Kodaki, Tsutomu | - |
dc.contributor.author | Makino, Keisuke | - |
dc.date.accessioned | 2021-09-08T18:49:03Z | - |
dc.date.available | 2021-09-08T18:49:03Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2009-03-25 | - |
dc.identifier.issn | 0168-1656 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/120404 | - |
dc.description.abstract | High stability of the oligonucleotides immobilized on the glass is essential for the reliable DNA microarray analysis. In the present study, effect of end-capping of the unreacted silanol, remaining after the surface amine-functionalization, was explored: (1) Cy3-NHS (N-hydorxysuccinincimide) dye was spotted on the surface and change in the fluorescent intensity was measured. (2) DNA probes were immobilized by the reactivity of oxanine linked at the 5'-end, the complementary oligonucleotides with Cy5-fluorescence at the 5'-end was hybridized, and the time-dependence of the fluorescence intensity was observed. Both the systems showed improved stability of the immobilized molecules, indicative of the stabilization by end-capping. (C) 2009 Published by Elsevier B.V. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | PERFORMANCE LIQUID-CHROMATOGRAPHY | - |
dc.subject | CARBON-TETRACHLORIDE | - |
dc.subject | COVALENT ATTACHMENT | - |
dc.subject | STATIONARY PHASES | - |
dc.subject | SILICA | - |
dc.subject | ALKYLSILANE | - |
dc.subject | EXPRESSION | - |
dc.subject | CATALYSTS | - |
dc.subject | OXANINE | - |
dc.subject | SURFACE | - |
dc.title | Stabilization of the immobilized linkers and DNA probes for DNA microarray fabrication by end-capping of the remaining unreacted silanol on the glass | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Pack, Seung Pil | - |
dc.identifier.doi | 10.1016/j.jbiotec.2009.01.004 | - |
dc.identifier.scopusid | 2-s2.0-62849121250 | - |
dc.identifier.wosid | 000265465500013 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOTECHNOLOGY, v.140, no.3-4, pp.242 - 245 | - |
dc.relation.isPartOf | JOURNAL OF BIOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF BIOTECHNOLOGY | - |
dc.citation.volume | 140 | - |
dc.citation.number | 3-4 | - |
dc.citation.startPage | 242 | - |
dc.citation.endPage | 245 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | PERFORMANCE LIQUID-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | CARBON-TETRACHLORIDE | - |
dc.subject.keywordPlus | COVALENT ATTACHMENT | - |
dc.subject.keywordPlus | STATIONARY PHASES | - |
dc.subject.keywordPlus | SILICA | - |
dc.subject.keywordPlus | ALKYLSILANE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CATALYSTS | - |
dc.subject.keywordPlus | OXANINE | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordAuthor | End-capping | - |
dc.subject.keywordAuthor | Amine-functionalized surface | - |
dc.subject.keywordAuthor | DNA microarray | - |
dc.subject.keywordAuthor | DNA immobilization | - |
dc.subject.keywordAuthor | Surface modification | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.