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An Electrochemical Biosensor Array for Rapid Detection of Alanine Aminotransferase and Aspartate Aminotransferase

Authors
Song, Min-JungYun, Dong-HwaHong, Suk-In
Issue Date
3월-2009
Publisher
TAYLOR & FRANCIS LTD
Keywords
glutamate sensor; alanine aminotransferase (ALT)/aspartate aminotraseferase (AST); activities; biosensor array; porous silicon; electrochemical biosensor
Citation
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.73, no.3, pp.474 - 478
Indexed
SCIE
SCOPUS
Journal Title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume
73
Number
3
Start Page
474
End Page
478
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/120488
DOI
10.1271/bbb.60043
ISSN
0916-8451
Abstract
An increment of alanine aminotransferase (ALT) or aspartate aminotransferase (AST) in human serum indicates an abnormal symptom of the liver. Hence, an electrochemical biosensor array that uses micro electromechanical systems technology is required for rapid and integrated measurement of ALT/AST. Here we describe a biosensor array consisting of two glutamate sensors. It turned out that porous silicon layers formed on each working electrode were useful to increase the effective surface area. This biosensor array was constructed with platinum electrodes and a polydimethylsiloxane (PDMS) microchannel. Electrodes in sampling wells minimized a cross-interference effect and permitted multiple sampling by immobilization with glutamate oxidase using a silanization technique. The device sensitivities derived from semi-logarithmic plots were 0.145 mu A/(U/l) for ALT and 0.463 mu A/(U/l) for AST over a range of 1.3 U/l to 250 U/l. Hene, this ALT/AST biosensor array can be applied in diagnostic and home use.
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College of Engineering > Department of Materials Science and Engineering > 1. Journal Articles
College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles

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